Application of RNA interference in triatomine （Hemiptera： Reduviidae） studies  

作　　者：Rafaela M. M. Paim Ricardo N. Araujo Michael J. Lehane Nelder F. Gontijo MarcosH. Pereira 

机构地区：[1]Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Bloco 14, Sala 177, Av. AntonioCarlos 6627, Pampulha, CEP 30270-901 Belo Horizonte, MG, Brazil [2]Liverpool School of Tropical Medicine, Pembroke Place, LiverpoolL3 5QA, UK

出　　处：《Insect Science》2013年第1期40-52,共13页昆虫科学（英文版）

摘　　要：Triatomines （Hemiptera： Reduviidae） are obligate hematophagous insects. They are of medical importance because they are vectors of Trypanosoma cruzi, the causative agent of Chagas disease in the Americas. In recent years, the RNA interference （RNAi） technology has emerged as a practical and useful alternative means of studying gene function in insects, including triatomine bugs. RNAi research in triatomines is still in its early stages, several issues still need to be elucidated, including the description of the molecules involved in the RNAi machinery and aspects related to phenotype evaluation and persistence of the knockdown in different tissues and organs. This review considers recent applications of RNAi to triatomine research, describing the major methods that have been applied during the knockdown process such as the double-stranded RNA delivery mechanism （injection, microinjection, or ingestion） and the phenotype characterization （mRNA and target protein levels） in studies conducted with the intent to provide greater insights into the biology of these insects. In addition to the characterization of insect biomolecules, some with biopharmacological potential, RNAi may provide a new view of the interaction between triatomine and trypanosomatids, enabling the development of new measures for vector control and transmission of the parasite.Triatomines （Hemiptera： Reduviidae） are obligate hematophagous insects. They are of medical importance because they are vectors of Trypanosoma cruzi, the causative agent of Chagas disease in the Americas. In recent years, the RNA interference （RNAi） technology has emerged as a practical and useful alternative means of studying gene function in insects, including triatomine bugs. RNAi research in triatomines is still in its early stages, several issues still need to be elucidated, including the description of the molecules involved in the RNAi machinery and aspects related to phenotype evaluation and persistence of the knockdown in different tissues and organs. This review considers recent applications of RNAi to triatomine research, describing the major methods that have been applied during the knockdown process such as the double-stranded RNA delivery mechanism （injection, microinjection, or ingestion） and the phenotype characterization （mRNA and target protein levels） in studies conducted with the intent to provide greater insights into the biology of these insects. In addition to the characterization of insect biomolecules, some with biopharmacological potential, RNAi may provide a new view of the interaction between triatomine and trypanosomatids, enabling the development of new measures for vector control and transmission of the parasite.

关 键 词：dsRNA KNOCKDOWN PHENOTYPE RNAi TRIATOMINE 

分 类 号：Q969.366.4[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]