HLA-DRB1*03/04等位基因与广西原发性肝癌家族聚集性的相关性  

Relationship between HLADRB1*03/04 alleles and familial clustering of hepatocellular carcinoma in Guangxi

作　　者：马莎莎[1] 吴继周[1] 吴健林[1] 刘芳[1] 胡蝶飞[1] 宁秋悦[1] 庞裕[1] 

机构地区：[1]广西医科大学第一附属医院感染性疾病科,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2013年第4期346-351,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30960170;广西卫生厅重点科研课题基金资助项目;No.桂卫重200924~~

摘　　要：目的:探讨HLA-DRB1*03/04等位基因与广西原发性肝癌家族聚集性的相关性,为寻找原发性肝癌的遗传易感基因或抗病基因提供线索.方法:采取性别、年龄±5岁配对方法,在广西肝癌高发区选取肝癌高发家族成员、肝癌单发家族成员和无癌家族成员各150例作为研究对象,采集研究对象外周全血提取DNA,应用PCR-SSP方法对HLA-DRB1*03/04等位基因进行检测.结果:HLA-DRB1*03在肝癌高发家族组的表达频率(16.7%)稍高于肝癌单发家族组(12.7%)及无癌家族组(16.0%),该等位基因在3组间的分布无显著性差异(?2=1.074,P=0.584);HLA-DRB1*04在肝癌高发家族组的表达频率(14.7%)稍高于肝癌单发家族组(5.3%)及无癌家族组(7.3%),其在3组间的分布有显著性差异(?2=8.748,P=0.013).结论:HLA-DRB1*04等位基因可能与广西肝癌高发区原发性肝癌的家族聚集性存在相关性;而HLA-DRB1*03等位基因则与之无明显相关.AIM：To investigate the relationship between HLA-DRB1＊03/04 alleles and familial clustering of hepatocellular carcinoma（HCC） to identify genes related to susceptibility to HCC in Guangxi,China.METHODS：One hundred and fifty members whose families have had two or more HCC patients were selected as the case group,and 150 members whose families have had only one HCC case and 150 members from families without any cancer were selected as controls.Peripheral blood samples were collected to extract DNA for testing HLA-DRB1＊03/04 alleles by PCR-SSP.RESULTS：The frequency of the HLA-DRB1＊03/04 alleles were all slightly higher in the case group than those in the two control groups.The difference in HLA-DRB1＊03 allele distribution among the three groups was not statistically significant（χ2=1.074,P=0.584）,but the difference in HLADRB1＊04 allele distribution was significant（χ2=8.748,P=0.013）.CONCLUSION：HLA-DRB1＊04 allele,not HLADRB1＊03 allele,might be associated with familial clustering of hepatocellular carcinoma in Guangxi,China.

关 键 词：HLA-DRB1＊03 04 等位基因 原发性肝癌 家族聚集性 

分 类 号：R735.7[医药卫生—肿瘤]