荧光原位杂交与染色体核型分析应用于自然流产病因学诊断的比较研究  

Comparative Study of Fluorescence in situ Hybridization Analysis and Karyotype Analysis in Spontaneous Abortion Etiology Diagnosis

作　　者：常亮[1] 张秋芳[1] 李丹[1] 赵楠[1] 刘平[1] 乔杰[1] 

机构地区：[1]北京大学第三医院妇产科生殖医学中心、教育部辅助生殖重点实验室、北京市生殖内分泌与辅助生殖重点实验室,北京100191

出　　处：《生殖与避孕》2013年第2期73-77,共5页Reproduction and Contraception

基　　金：国家自然基金资助,项目号:81170538

摘　　要：目的：分析并比较荧光原位杂交技术（fluorescence in situ hybridization，FISH）TL普通染色体核型分析技术在自然流产中的诊断意义。方法：以早孕自然流产的患者为研究对象，共201例。将同一孕周的患者随机分为A组和B组，A组（”=100）进行绒毛培养加染色体核型分析，B组（n=101）进行FISH分析，另在A、B组孕6-11周患者中每一孕周各随机选取1例，每组6例，共12例同时进行2种技术分析，并比较结果。结果：染色体核型分析成功率为66％，其中核型异常率为30．3％；FISH成功率为100％其中核型异常率为46．5％；2种检测技术检测出的异常核型率比较有统计学差异（P=0．036）。结论：2种分析技术对异常核型的检出率有明显的差异，FISH更容易成功，更能反应胚胎的染色体数目；染色体核型分析结合FISH技术能有效诊断自然流产的染色体异常。Objective： To compare the roles of fluorescence in situ hybridization （FISH） and karyotype analysis on chorionic villus in spontaneous abortion. Methods： A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The uncultured villi from 101 women （group B） were used for FISH, while the villi from 100 cases （group A） were cultured and used for karyotype analysis. A case was randomly selected from one gestational week （6-1 lweeks） pregnant patients from each group, 6 cases from each group and a total of 12 cases were analyzed by FISH and karyotype at the same time. Results： The successful karyotype analysis rate was 66%, and the abnormality karyotype rate was 30.3%; FISH successful analysis rate was 100%, and the abnormality rate was 46.5%; there were significant differences between FISH and karyotype analysis （P=0.036）. Conclusion： There were obvious differences between the two techniques. FISH was more successfully analyzed, and was used to more precisely determine fetal chromosome number. It is an effective way to determine abnormal chromosome by integrating FISH and karyotype analysis in spontaneous abortion.

关 键 词：自然流产 早期妊娠 荧光原位杂交(FISH) 核型分析 

分 类 号：R714.21[医药卫生—妇产科学]