海带多糖对人鼻咽癌HONE1细胞裸鼠移植瘤的抑制及凋亡相关基因的调控  

Regulation to apoptosis-related genes of human nasopharyngeal carcinoma HONE1 cell xenografts in nude mice by laminaria japonica polysaccharides

作　　者：孙文忠[1,2] 魏媛媛[1] 曾曼丽[1] 郑实兴[1] 徐志文[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻喉-头颈外科,南宁市530021 [2]广西柳州市人民医院耳鼻喉-头颈外科

出　　处：《实用医学杂志》2013年第4期535-537,共3页The Journal of Practical Medicine

基　　金：广西科学基金项目资助(编号:桂科自0728241)

摘　　要：目的:观察海带多糖(laminaria japonica polysaccharides,LJP)对人鼻咽癌(nasopharyngeal carcinoma,NPC)HONE1细胞移植瘤生长抑制及凋亡相关基因调控效应,探讨其抗癌机制。方法:以人NPC细胞株HONE1建立裸鼠皮下移植瘤模型及LJP行体内抑瘤实验,并以RT-PCR检测移植瘤Bax,Bcl-2,Caspase-3,8,9mRNA表达。结果:LJP中、高剂量组抑瘤率分别为33.7%和47.0%,具有明显抑瘤作用;RT-PCR检测结果显示,Bax mRNA表达随着LJP浓度的增加而增高(P<0.05);Bcl-2mRNA表达随着LJP浓度增加而降低(P<0.05)。Caspase-3mRNA表达随着LJP浓度增加而增高(P<0.05);LJP组Caspase-8,9mRNA表达无差异,且Caspase-9mRNA表达均低于顺铂(cisplatin,DDP)组。结论:LJP具有抑制NPCHONE1细胞生长的作用,其机制是促进癌细胞凋亡而实现的。Objective This study aims to evaluate the antitumor activity of laminaria japonica polysaccharides （LIP） and its effect on apoptosis-related genes to human nasopharyngeal carcinoma HONE1 cell xenografts in nude mice and further to explore the underlying mechanism. Methods A tumor model, established by subcutaneously inoculation of NPC cell HONE1 into nude mice,was used to evaluate the antitumor effect of IJP in vivo, which were obsenTed to detect the expression of Bax, Bcl-2,Caspase-3,8,9 messenger RNA （mRNA） with LIP drug intervention by RT-PCR. Results The growth of xenografts in nude mice was significantly suppressed after administration of LJP at a dose-dependent manner. The inhibition rates were 33.7% and 47.0% when treated at medium and high dose respectively. RT-PCR test results show, Bax mRNA expression increased with increasing LIP concentration （P 〈 0.05 ） ; Bel-2mRNA expression decreased with increasing LJP concentration （P 〈 0.05 ）.With LIP concentration increasing,the Caspase-3mRNA expression increased （P 〈 0.05） ; Caspase-8,9mRNA expression in LJP groups no difference, And Caspase-9mRNA expression was lower than that in DDP group. Conclusion LIP can inhibit the growth of NPC HONE1 cell lines, probably by inducing apoptosis of NPC HONE 1 lines ceils in vivo.

关 键 词：鼻咽肿瘤 海带多糖 移植瘤 逆转录聚合酶链式反应 凋亡 

分 类 号：R739.63[医药卫生—肿瘤]