纳秒级陡脉冲治疗皮下移植瘤安全性研究  

Safety Evaluation of Nanosecond Pulsed Electric Field in Treatment of Subcutaneously Transplanted Tumor

作　　者：夏如民[1] 赵雪[2] 唐均英[2] 郭飞[3] 王建[3] 姚陈果[3] 

机构地区：[1]怀化市第一人民医院,怀化418000 [2]重庆医科大学附属第一医院妇产科,重庆400016 [3]重庆大学电气工程学院高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《世界科技研究与发展》2013年第1期118-121,共4页World Sci-Tech R&D

基　　金：国家自然科学基金重点项目(50637020)资助

摘　　要：目的研究纳秒级陡脉冲治疗皮下移植瘤的安全性。方法建立32只裸鼠人恶性黑色素瘤皮下移植瘤动物模型,随机分为实验组(n=16)和对照组(n=16),实验组采用场强为40 kV/cm、脉宽200 ns、频率1 Hz、脉冲个数1 000个的脉冲电场处理肿瘤,观察处理后荷瘤鼠一般情况及生存期,热电偶温度计测量治疗前后肿瘤即刻温度变化,彗星拖尾实验检测治疗后肿瘤周边组织DNA损伤情况,电镜观察裸鼠肝、肾组织变化。结果与对照组比较,经纳秒级陡脉冲处理后,裸鼠一般状况良好,处理处皮肤形成溃疡后未经处理能自行愈合,治疗组生存期(60.6±6.9 d)较对照组(17.7±5.4 d)明显延长(P<0.01),生存期延长率为242%。经纳秒级陡脉冲处理后的肿瘤前后即刻温度变化小,治疗后肿瘤周边组织DNA未见彗星拖尾现象发生,说明无DNA损伤发生,肝肾组织HE染色未见明显改变。结论纳秒级陡脉冲治疗皮下移植瘤是安全的。Objective To evaluate the safety of nanosecond pulsed electric field （ nsPEF ） in the treatment of subcutaneously transplanted tumor. Methods The models of human melanoma A375 cell were established in 32 BALB/e female nude mice, and all animals were divided into two groups randomly ：the nsPEF group （ n = 10） and the control group （ n = 10 ）. nsPEF group tumors were exposed to the 40KV/cm, 200ns, 1Hz, 1000 pulses, general condition and survival time after nsPEFs treatment were monitored. Measurement of the tumor temperature instantly change before and after usPEF treatment. Comet assay was used to detect the normal tissues surrounding the tumor. The Pathological of the liver and kidney were detected by HE staining. Results Compared with the control group, nude mice generally in good condition, pro- cessing at the untreated skin and the formation of ulcers can heal itself, The mean survival time was significantly longer in nsPEF group （60.6 ± 6.9 d） than in control group（ 17.7 ± 5.4 d） （ P 〈 0.01 ）. The increase ratio of life span reached up to 242%. We directly measured this temperature increase a very small by thermocouple. No comet tailing was observed, indicating that no DNA lesions after nsPEF treatment. The Pathological of the liver and kidney were no significant change. Conclusion The nsPEF is verified to be safe.

关 键 词：脉冲电场 纳秒级陡脉冲 安全性 

分 类 号：R739.5[医药卫生—肿瘤]