超声波强化提取杜氏盐藻中β-胡萝卜素工艺的研究  

Ultrasound-assisted Extracting Techniques of β-carotene from Dunaliella salina

作　　者：周鸣谦[1] 刘云鹤[1,2] 陈宏柱 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]江苏省海洋生物技术重点实验室,江苏连云港222005

出　　处：《食品研究与开发》2012年第12期54-57,共4页Food Research and Development

基　　金：江苏省产学研联合创新项目(BY2010127);江苏省海洋生物技术重点实验室开放课题(2009HS05);淮海工学院自然科学基金项目(Z2007038)

摘　　要：对超声波强化提取杜氏盐藻中β-胡萝卜素的工艺进行了研究,以β-胡萝卜素的得率为评价指标,在单因素试验基础上,通过四因素三水平正交试验设计确定了提取温度、提取时间、超声波强化时间和液料比4个因素共同影响下,从杜氏盐藻中提取β-胡萝卜素的最优工艺条件,即液料比6∶1(mL∶g),超声波强化时间70 s,提取温度20℃,提取时间为9 min。在上述提取条件下,β-胡萝卜素的得率可达到4.418%.The ultrasound-assisted extracting techniques of β-carotene from Dunaliella salina were studied and optimized in this experiment,in which the extraction amount of β-carotene from Dunaliella salina was the evaluation indicator.The optimum extraction conditions were obtained by orthogonally designed experiments and single factor trials.The experiment result showed that the best extraction conditions of β-carotene from Dunaliella salina were as follows.Organic solvent quantity was 6∶1（mL∶g）,time of ultrasound-assisted was 70 seconds,extraction temperature was 20 ℃ and extraction time was 9 minute.Under above conditions,the extraction amount of β-carotene from Dunaliella salina was 4.418 %.

关 键 词：杜氏盐藻 Β-胡萝卜素 超声波 提取 

分 类 号：TQ914.2[化学工程]