重组黑曲霉产大豆异黄酮糖苷酶发酵条件的研究  

udy on Soybean Isoflavone-glucosidase Fermentation Conditions of the Recombinant by Aspergillus Niger

作　　者：王丹[1,2] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]长春医学高等专科学校,吉林长春130031

出　　处：《食品研究与开发》2012年第12期172-174,共3页Food Research and Development

摘　　要：通过单因素试验和正交试验,探讨野生型黑曲霉重组菌株产大豆异黄酮糖苷酶的最佳条件。结果表明:在培养基组分为麸皮4%、玉米芯2%、豆饼粉2%、NH4Cl 2%、KH2PO40.1%,pH为6.0,接种量为10%,培养温度为28℃,72 h的条件下培养可获得较高的产酶活性,可达2.463×1010kat/mL。The optimal conditions for recombinant of aspergillus niger producing soybean isoflavone-glyco-sidase were determined by the single factor and orthogonal test experiment.The results showed that the medium was composed of 4 % bran,2 % corncob,2 % bean cake powder,2 % NH4Cl,0.1 % KH2PO4.The inoculums was 10 %,pH6.0 and the cultivation period was 72 h at 28 ℃.The activity of Aspergillus niger was 2.463×1010 kat/mL.

关 键 词：黑曲霉 重组菌株 大豆异黄酮糖苷酶 产酶条件 

分 类 号：TS201.2[轻工技术与工程—食品科学]