6种海洋微藻β-葡聚糖的累积特征及其免疫活性分析  

Accumulation Features and Immuno-stimulative Effects of β-Glucans from Six Species of Marine Microalgae

作　　者：田甜[1] 周成旭[1] 刘宝宁[1] 陈海敏[1] 严小军[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《食品科学》2013年第3期188-192,共5页Food Science

基　　金：教育部"长江学者和创新团队发展计划"项目(IRT0734);国家自然科学基金项目(31172448);浙江省重点基金项目(Z3100565);浙江省自然科学基金项目(LY12D06001);海洋可再生能源专项资金项目(GHME2001SW02);国家公益性行业(海洋)科研专项(201105009)

摘　　要：跟踪检测6种海洋微藻在生长过程中单位细胞β-葡聚糖的含量变化,分析其累积时期、累积速率以及提取制备的得率和纯度,并对几种微藻来源的β-葡聚糖进行免疫活性检测。结果表明:β-葡聚糖在微藻种群增殖的平台期累积量最大,不同种类的微藻细胞累积β-葡聚糖能力差异较大。假微型海链藻(Thalassiosira pseudonana)具有较大的累积速率、提取制备得率以及较大的免疫活性。6种海洋微藻来源的β-葡聚糖都显示比酵母来源的β-葡聚糖免疫活性高。The accumulation features of microalgal fl-glucans, including accumulation phase and rate, have been characterized by monitoring the content of β-glucans from six marine microalgae during their growth cycles. The extraction and immuno-stimulative effects of β-glucans were also studied. The results indicated that the content of cellular β-glucans reached the maximum level at stationary phase for all microalgae, however, with large variation depending on species. Among six microalgae, Thalassiosira pseudonana showed outstanding features for its β-glucans with high accumulation rate, isolation yield, and significant immuno-stimulative effects. Therefore, the immuno-stimulative effects of β-glucans were higher from microalgae than that from yeast.

关 键 词：海洋微藻 Β-葡聚糖 累积效率 提取率 免疫活性 

分 类 号：Q945.79[生物学—植物学]