复合菌剂解无机磷条件优化研究  

Optimizing the Phosphorus-solubilizing Condition of Multiple Species Inoculants

作　　者：侯佳奇[1] 李鸣晓[2] 贾璇[2] 席北斗[2] 魏自民[1] 党秋玲[2] 宋彩红[2] 赵国鹏[1] 

机构地区：[1]东北农业大学生命科学院,哈尔滨150030 [2]中国环境科学研究院,北京100012

出　　处：《农业环境科学学报》2013年第2期385-392,共8页Journal of Agro-Environment Science

基　　金：国家科技支撑计划项目村镇有机废弃物高值化分质利用技术集成;装备研发(2012BAJ21B02)

摘　　要：研究采用单纯形重心设计方法对3株耐高温解无机磷菌株(P1、P2、P3)进行了复合功能菌剂的组合,并通过响应曲面法(RSM)建立了耐高温功能复合菌剂解磷条件的二次多项数学模型,对复合菌剂的解磷条件进行优化。对7组实验水平的分析结果表明:组合4(P1:P2:P3=0:50%:50%)各个菌株间能够相互促进,协同共生效果最佳,解磷量为241.70mg·L-1,显著高于各单株菌与其他组合。中心组合实验Box-Behnken对影响复合功能菌剂解磷量的5个关键因素[培养时间(x1)、培养温度(x2)、pH(x3)、磷矿粉添加量(x4)、接种量(x5)]的最佳水平范围及其交互作用进行设计。试验数据多元拟合结果表明:(1)培养时间一次项、pH一次项、培养时间和pH交互项、培养时间和磷矿粉添加量交互项、温度和磷矿粉添加量交互项、以及所有二次项均达到极显著水平(P<0.01)。(2)培养时间、pH对复合功能菌剂解磷量影响较强,其他因素的影响效果由大到小依次为接种量、磷矿粉添加量、温度。(3)优化得到耐高温复合功能解磷菌剂最佳解磷条件为培养时间7.18d,培养温度49.98℃,pH6.63,磷矿粉添加量6.21g·L-1,接种量6.07%。验证试验表明,响应曲面法可以科学有效构建复合菌剂解磷条件的数学模型,合理优化解磷条件的影响因素。Three strains of thermophilic phosphate solubilizing microorganisms named P1 , P2 , P3 , were selected and mixed as multiple species inoculants(MSI) according to Simple-centroid method with Design Expert software. Then quadratic polynomial mathematical model of the solubilized-phosphorus condition of thermophilic MSI was established by response surface methodology(RSM) . Compared with seven levels, the results showed that the Phosphate-solubilizing quantity of P 1:P 2:P 3 =0:50%:50%(241.70mg·L-1) level was significantly higher than other MSI. Based on the design of Box-Behnken to optimize the optimal levels and the interaction of five important factors which included the incubation time(x1) , the incubation temperature(x2) , pH(x3) , the content of rock phosphate(x4) , the inoculum size of microorganism(x5) . The results showed that:(1) Linear term of incubation time and pH, interaction term of incubation time and pH, interaction term of incubation time and content of rock phosphate, interaction term of incubation temperature and content of rock phosphate, quadratic term of all the variable were reached extremely significant leve(l P<0.01).(2) Incubation time and pH were the key factors on solubilized-phosphorus capability using MSI. The influence of other factors was the inoculum size of microorganism>rock phosphate proportion>temperature.(3) The optimized result of the solubilized-phosphorus condition of thermophilic compound bacterium was as follows:the inoculum size of microorganism was 6.07%, and the content of rock phosphate was 6.21 g·L-1 with the incubation temperature at 49.98℃ for 7.18 d under 6.63 pH value. Verification tests show that the response surface methodology can scientific and effective build a thermophilic compound bacterium model, reasonably optimize the influencing factors of the solubilizing conditions.

关 键 词：耐高温解无机磷菌 解磷条件 响应曲面法 单纯形重心设计 

分 类 号：X172[环境科学与工程—环境科学]