绿茶多酚对帕金森病小鼠多巴胺能神经元保护作用研究  

Protective effect of green tea polyphenols on substantia nigra dopaminergic neurons of MPTP induced Parkinson′s disease mice

作　　者：陈敏[1] 黄雪芳 叶俊[3] 于兰[1] 刘承伟[1] 

机构地区：[1]桂林医学院人体解剖学教研室,广西桂林541004 [2]广西壮族自治区桂林市南溪山医院药剂科,541002 [3]桂林医学院附属医院急诊科,广西桂林541001

出　　处：《重庆医学》2013年第7期721-723,共3页Chongqing medicine

基　　金：广西自然科学基金资助项目(2012GXNSFBA053110);广西科学研究与技术开发项目(10124002B-58);广西卫生医疗重点科研项目(重2011012);广西高等学校一般资助科研项目(201203YB115);广西高等学校立项科研项目(201204LX253);桂林市科学研究与技术开发项目(20100511)

摘　　要：目的探索绿茶多酚(GTPs)对神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)小鼠黑质多巴胺能神经元预防性保护作用及机制。方法采用C57BL/6J小鼠共60只,分为空白对照组、预防组、MPTP组和治疗组(n=15)。MPTP组以MPTP腹腔注射7d(30mg.kg-1.d-1);预防组同以上述剂量注射MPTP前3个月开始GTPs干预至MPTP处理后3个月;治疗组以相同MPTP处理后开始GTPs干预3个月;空白对照组:腹腔注射同体积的生理盐水,连续7d,饮水中未加入GTPs,余处理方法及时长同治疗组。免疫组织化学法观察黑质多巴胺能神经元计数,比色法观察中脑超氧化物歧化酶(SOD)和丙二醛(MDA)反映氧化应激水平。结果预防组、MPTP组和治疗组黑质多巴胺能神经元数量显著减少(P<0.05),氧化应激水平明显升高(P<0.05)。结论预防性使用GTPs对PD小鼠的多巴胺能神经元具有高于治疗的保护作用,其机制可能为预先增强抗氧化应激能力,提高发生病理改变的阈值。Objective To investigate the preventive protection effect of green tea polyphenols（GTPs） on substantia nigra do- paminergic neurons in 1-methyl-l-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP） induced Parkinsonr s disease mice and its mecha- nism. Methods C57BL/6J mice（n= 60） were divided into the blank control group, prevention group, MPTP group and treatment group equally. The MPTP group was given with MPTP by intraperitoneal injection for 7 d（30 mg · kg-1 · d-1 ）. The prevention group began GTPs intervention at 3 months before injection of MPTP with above dose until 3 months after MPTP treatment. The treatment group began GTPs intervention for 3 months after the same MPTP treatment. The blank control group was intraperitone- ally injected with the same volume of normal saline for continuous 7 d without adding GTPs to drinking water,the other treatment measures and duration were same to the treatment group. The immunohistocbemical was adopted for detecting the number of sub- stantia nigra dopaminergic neurons. Superoxide dismutase（SOD） and malonaldehyde（MDA） in midbrain were determined by colori- metry,which reflecting the oxidative stress level. Results The number of substantia nigra dopaminergic neurons in the prevention group,MPTP group and treatment group was significantly decreased（P〈0.05）, and the oxidative stress level was obviously in- creased（P〈0.05）. Conclusion Preventive use of GTPs has the protective role on the dopaminergic neurons of Parkinson＇s disease mice,which is higher than treatment. The possible mechanisms are to enhance the antioxidant stress ability in advance and increase the threshold value of pathological change.

关 键 词：帕金森病 绿茶多酚 预防保护 多巴胺能神经元 氧化应激 

分 类 号：R742.5[医药卫生—神经病学与精神病学]