产木聚糖酶菌种的筛选及其在纸浆漂白中的应用  

The Sifting of Strains that Producing Xylanas-eand Its Application in Pulp Bleaching

作　　者：卢庆华[1] 卢俊[2] 蔡禄[1] 

机构地区：[1]内蒙古科技大学生物工程与技术研究所,包头014010 [2]包头轻工职业技术学院,包头014035

出　　处：《科学技术与工程》2013年第5期1257-1260,1275,共5页Science Technology and Engineering

基　　金：教育部春晖计划(Z200721201020);内蒙古自治区高等学校科学研究项(NJzy08233);内蒙古包头市科技攻关项(2008y100222)资助

摘　　要：筛选出高产木聚糖酶的菌株,并将其应用于纸浆漂白。采用乙醇水解圈法筛出一株产木聚糖酶较高的菌种,并对其酶学性质进行了研究,同时对菌种做了初步鉴定,进一步探讨了其酶液在纸浆漂白中的应用。研究结果表明筛选得到的菌种酶活最高可达642 U/mL,经初步鉴定为米曲霉;菌体最适产酶温度为50℃,酶活最适温度为50℃,pH为5～6;当木聚糖酶用量为6U/mL时,效果达到最佳,卡伯值由32.3降低至25.5,白度由37.2提高到48.7,提高约31%。The strains of high yield xylanase used in pulp bleaching were screened. A strain produced high xylanase was gained according to the ethanol hydrolysis circle method, and.its enzymatic properties were studied.Meanwhile, strains was made a preliminary identification, and enzyme in pulp bleaching applications was discussed. The results show that the screened bacteria activity was up to 642 U / mL, and was identified as one of Aspergillus oryzae. The optimal temperature of bacterial producted enzyme was 50癈, the best temperature of the activity is 50癈,too, and pH was 5 to 6. The effect was best that kappa number was reduced from 32.3 to 25.5, and the whiteness increased significantly when xylanase dosage was 6U/ml.

关 键 词：筛选 木聚糖酶 酶学性质 纸浆漂白 

分 类 号：Q819[生物学—生物工程]