维吾尔族和汉族直肠癌患者K-ras基因第12、13位密码子突变情况的研究  

Investigation of K-ras Gene Codon 12 and 13 Mutations in The Uyghur and Han Patients with Rectal Cancer

作　　者：付大鹏[1,2] 龚旭晨[2] 孔长青[3] 

机构地区：[1]安徽医科大学新疆临床学院 [2]新疆维吾尔自治区人民医院肛肠外科,新疆乌鲁木齐830000 [3]新疆维吾尔自治区人民医院普外二科,新疆乌鲁木齐830000

出　　处：《中国普外基础与临床杂志》2013年第2期165-168,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨新疆地区维吾尔族和汉族直肠癌患者K-ras基因外显子2中第12、13位密码子的突变情况,为新疆地区直肠癌患者的临床及基因诊断提供理论参考。方法收集2010年1月至2011年12月期间在新疆维吾尔自治区人民医院手术切除的临床资料完整的直肠腺癌石蜡包埋组织144例,同时选取距癌缘近端10 cm以远的正常直肠石蜡包埋组织144例作为对照组。提取癌组织DNA,经聚合酶链反应扩增K-ras基因,采用DNA直接测序法检测K-ras基因的突变情况。结果 144例直肠癌患者中有32例(22.22%)K-ras基因第12、13位密码子发生突变,汉族与维吾尔族突变率分别为20.41%(20/98)和26.09%(12/46),二者比较差异无统计学意义(P>0.05)。对照组中均未见K-ras基因第12、13位密码子的突变。K-ras基因突变仅与肿瘤浸润深度有关(T1+T2为25.0%,T3+T4为75.0%,P=0.01),与民族、性别、肿瘤部位、分化程度及淋巴结转移均无关(P>0.05)。结论在维吾尔族与汉族直肠癌患者中K-ras基因第12、13位密码子突变均很常见,但在突变频率上维吾尔族与汉族直肠癌患者间无差异,K-ras基因突变与直肠癌的侵犯深度有关。Objective To investigate K-ras gene exon 2 codon 12 and 13 mutations, and analyze the clinical significance in the Uyghur and Han patients with rectal cancer. Methods A total of 144 surgical specimens taken from patients with rectal cancer who were treated in this hospital from January 2010 to December 2011 were used in this study. DNA was picked up from the tumor tissues and amplificated by PCR then direct sequencing. Results The K-ras gene mutation rate was 22. 22% （32/144）, which was 26. 09% （12/46） and 20.41% （20/98） in the Uyghur and Han patients, respectively, the difference was not statically significant （P〉0. 05）. The K-ras gene mutation was related to the depth of invasion （T1 ＋T2： 25.0%, T3＋T4： 75.0%, P=0. 01）, which was not related to the nation, gender, location of tumor, differentiation degree, or lymph node metastasis （P〉 0. 05）. Conclusions K-ras gene mutation is a common event in the Uyghur and Han patients with rectal cancer, but the K-ras gene mutation rate is not significant difference between the Uyghur nationality and Han nationality, which is only related to depth of invasion.

关 键 词：直肠癌 K-RAS基因 基因突变 

分 类 号：R735.37[医药卫生—肿瘤]