食管癌组织中p16基因突变及其产物表达的研究  

EXPRESSION AND MUTATION OF p16 GENE AND ITS PRODUCTS IN HUMAN ESOPHAGEAL SQUAMOUS CELL CARCINOMAS

作　　者：曹巍[1] 吕桂泉[2] 陈旭峰[2] 常扶保 王慧珍 郑树[5] 

机构地区：[1]新乡医学院病理教研室,新乡453003 [2]浙江省肿瘤医院 [3]河南省林县食管癌医院 [4]河南省新乡市中心医院 [5]浙江医科大学肿瘤所

出　　处：《肿瘤》2000年第3期191-194,F003,共5页Tumor

基　　金：河南省自然科学基金资助课题!(954020900)

摘　　要：目的　探讨p16基因变异及其产物表达与食管癌生物学行为的关系。方法　应用PCR SSCP和PCR方法检测了林县食管癌标本中p16 CDKN2 基因 1、2外显子的突变和缺失状况。并用免疫组化方法检测其产物表达情况。结果　在 47例标本中 ,3例出现了突变 ,其中第 1外显子 1例 ,第 2外显子 2例 ,突变率为 6 4% ( 3 47)。有 12例出现纯合性缺失 ,缺失率为 2 5 5 % ,其总变异率为 31 9%。免疫组化染色显示 ,47例食管癌组织中 2 0例出现 p16蛋白的表达 ,阳性率为 48 8%。结论　结论 p16基因变异可能在食管癌的发生中发挥一定的作用。p16蛋白的表达与食管癌的组织学分级之间有一定的关系 。Objective To investigate tbe relationship between p16 aberration,p16 protein and biological behavior of esophageal squamous cell carcinoma(ESCC).Methods Singlestrand conformation polymorphism(SSCP)and PCR analysis were used to detect mutation in exon 1,2 of the p16 gene and deletion of p16 gene.Multiclonal antibody was applied immunohistochemically to detect accumulation of p16 protein.Results Mutations were found in 3 of 47(64%) tumor samples,one in exon 1 and two in exon 2 Deletions of p16 gene were detected in 12 of 47(255%).The p16 aberration rate was 319%.Immunostaining of p16 protein was detected in 20 of 47 tumors(488%).A significantly higher rate of p16 protein expression was seen in cases with well differentiation than in cases with middellow differentiation.Conclusion These data suggest that p16/CDKN 2 gene alterations may play a role in the progression of human ESCC.The p16 protein expression could not independently predict prognosis in ESCC.$$$$

关 键 词：食管肿瘤 P16基因 基因突变 基因表达 

分 类 号：R735.102[医药卫生—肿瘤]