卵巢分化型上皮肿瘤端粒酶hTRT基因、p53、C-erb-B2蛋白检测与比较  

Expression of telomerase hTRT in differentiated ovarian epithelial tumors —comparison with alteration of p53 and c-erb-B2

作　　者：卫建平[1] 张波[2] 沈容 李圣爱[2] 侯琳[2] 

机构地区：[1]山西医科大学第二附属医院病理科,030001 [2]北京医科大学病理学系

出　　处：《诊断病理学杂志》2000年第1期33-35,I012,共3页Chinese Journal of Diagnostic Pathology

摘　　要：目的　探讨检测hTRT基因表达在卵巢分化型上皮源肿瘤中的价值。方法　用原位杂交检测 75例卵巢良性 (43例 )、低度恶性 (8例 )、恶性上皮源肿瘤 (2 4例 )hTRT基因表达 ,同时用免疫组化检测p5 3,C erb B2蛋白表达。结果　 75例肿瘤中 ,hTRT基因 :良性肿瘤 12 % (5 / 43) ;低度恶性肿瘤 38% (3/ 8) ;恶性肿瘤 96 % (2 3/ 2 4) ,三者间存在着显著性差异 (P <0 0 5 ) ,淋巴结转移性浆液性乳头状囊腺癌 10 0 % (2 / 2 )。p5 3阳性分布 :良性肿瘤 0 % (0 /43) ;低度恶性肿瘤 2 5 % (2 / 8) ;恶性肿瘤 42 % (10 / 2 4) ,三者间存在着显著性差异 (P <0 0 5 )。淋巴结转移性浆液性乳头状囊腺癌 5 0 % (1/ 2 )。C erb B2阳性分布 :良性肿瘤 9% (4/ 43) ;低度恶性肿瘤 2 5 % (2 / 8) ;恶性肿瘤 46 % (11/2 4)。良性与恶性肿瘤间存在着显著性差异 (P <0 0 5 ) ,而良性与低度恶性间无差异 (P >0 0 5 )。hTRT与p5 3,C erb B2的表达均无相关性 (P >0 0 5 )。结论　端粒酶hTRT基因在恶性卵巢分化型上皮源肿瘤中有显著性改变 ,其基因的表达较p5 3,C erb B2蛋白改变更为普遍 。Objective To evaluate detection of telomerase hTRT expression in ovarian epithelial tumor . Methods Total 75 cases of ovarian epithelial tumors , including 43 cases of benign tumors , 8 borderline and 24 malignant tumors, were analyzed with in situ hybridization and immunohistochemistry. Results Expression of hTRT was positively detected in 12% (5/43) benign ,38%(3/8) borderline and 96%(23/24) malignant tumors. A significant difference among the three groups was found ( P <0.05) . The p53 positivity was distributed in 0%(0/43) benign, 25%(2/8) borderline and 42%(10/24) malignant tumors; The significant difference was also showed in the three groups ( P <0.05). The positivity of c erb B2 was 9%(4/43) for benign, 25%(2/8) borderline and 46%(11/24) malignant tumors; The difference was only demonstrated between benign and malignant groups. No correlation of expression was found among hTRT , p53 and c erb B2 ( P >0.05). Conclusions Expression of telomerase hTRT was significantly correlated with the malignancy of ovarian epithelial tumors, and more frequent positive than p53 and c erb B2. It is suggested that hTRT expression may be a good marker in diagnosis of malignant ovarian epithelial tumors.

关 键 词：HTRT C-ERB-B2 P53 卵巢肿瘤 分化型上皮肿瘤 

分 类 号：R737.310.2[医药卫生—肿瘤]