反相高效液相色谱法测定植物油中苯并(a)芘含量  

Determination of benzo(a)pyrene in vegetable oil by reverse-phase high performance liquid chromatography

作　　者：田玉霞[1] 

机构地区：[1]国家粮食储备局西安油脂科学研究设计院,西安710082

出　　处：《粮食与食品工业》2013年第1期61-62,65,共3页Cereal & Food Industry

摘　　要：将适量的试样溶解于石油醚中,用中性氧化铝柱净化,用石油醚洗脱苯并(a)芘,荧光检测器检测。结果显示,苯并(a)芘色谱峰面积与质量浓度在0～50μg/L的范围内呈良好的线性关系(r=0.999 5);方法的最低检测限为0.2μg/kg。苯并(a)芘的回收率为88.3%～98.5%。证明该方法准确、灵敏、快捷,建立了一种反相高效液相色谱法测定植物油中苯并(a)芘含量的方法。Food samples were dissolved in light petroleum, adsorbed on alumina columns, eluted with light petroleum to remove any benzo（a）pyrene present and determined by HPLC using fluorescence detection. Results show that the linear relationship between peak area and mass con- centration of benzo（a）pyrene was obtained at the range of 0-50 g/L（r = 0. 999 5）. The detection limit was 0.2 μg/kg and recovery was 88.3%-98. 5%. It has proved that the method is accurate, sensitive and rapid and a method of reversed--phase high performance liquid chromatography （RP--HPLC） for determination of benzo（a）pyrene in animal and vegetable fats and oils has been established.

关 键 词：苯并(A)芘 植物油 反相高效液相色谱法 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]