机体脱水对Lipocalin-2基因影响的时效关系研究  

The effects of dehydration on gene transcription of lipocalin-2 in rats

作　　者：王宇[1] 曾婷婷 

机构地区：[1]四川大学华西医院肿瘤分子诊断研究室,生物治疗国家重点实验室,四川成都610041 [2]成都市药品不良反应监测中心

出　　处：《现代预防医学》2013年第5期927-928,935,共3页Modern Preventive Medicine

摘　　要：目的研究在不同的脱水时间下,大鼠脑组织视上核中Lipocalin-2基因的变化情况。方法 60日龄雄性SD大鼠40只,随机分为脱水1d、2d、3d组和正常饮水对照组。脱水期满后处死动物,取脑组织视上核部分,采用RT-PCR和Western-blot检测Lipocalin-2的表达。结果与对照组相比,脱水2d、3d组视上核组织中Lipocalin-2的mRNA和蛋白表达水平都显著升高(P﹤0.05)。结论随着脱水时间的延长,Lipocalin-2在视上核组织中的含量逐渐增加,具有明显的时间-效应关系。提示了在机体脱水等原因导致的体液代谢混乱情况下,Lipocalin-2可能具有重要的生物学功能。OBJECTIVE To study the gene expression change of Lipocalin-2 in normal and water deprivated SD rat brain. METHODS 40 60d-old male SD rats were detached to control and three experimental groups randomly. Normal rat food and water was administrated to control group while experimental group was deprivated for normal water intake for ld, 2d and 3d. All animals were sacrificed in 4h, and SONs were taken from the whole brain. The amount of mRNA and protein expression of Lipocalin-2 was detected. RESULTS Gene transcription and protein expression of Lipocalin-2 in 2d and 3d experimental groups were obviously stronger than control group （P 〈 0.05）. CONCLUSION In chronic dehydration model, expression of Lipocalin- 2 is obviously up-regulated which indicates that exclusive role of Lipoealin-2 in serum osmoregulation, a significant time-effi- ciency relation is found and the results are further verified by previous proteomic study.

关 键 词：Lipocalin-2 渗透压调节中枢 视上核 

分 类 号：R394.3[医药卫生—医学遗传学]