虾青素对辛硫磷所致小鼠DNA损伤的拮抗作用  

Antagonistic effect of astaxanthin on DNA damage induced by phoxim in mice

作　　者：闫建国[1] 周亚莉[1] 邢雪琨[2] 王松涛[1] 方方[1] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院生命科学技术学院

出　　处：《环境与健康杂志》2013年第2期132-134,共3页Journal of Environment and Health

基　　金：新乡医学院高学历人才科研启动项目

摘　　要：目的探讨虾青素对辛硫磷所致小鼠遗传毒性的拮抗作用。方法将50只健康SPF级昆明小鼠随机分为5组,分别为对照组和80 mg/kg辛硫磷染毒组以及低、中、高剂量虾青素保护组(2 mg/kg、4 mg/kg、8 mg/kg),每组10只,雌雄各半。早晨低、中、高剂量虾青素保护组分别灌胃染毒2、4、8 mg/kg虾青素,对照组和辛硫磷染毒组灌胃染毒橄榄油,染毒容量为10 ml/kg;间隔约8 h后,下午低、中、高剂量虾青素保护组和辛硫磷染毒组分别灌胃染毒80 mg/kg辛硫磷,对照组灌胃生理盐水,染毒容量为10 ml/kg,连续染毒14 d。采用微核实验检测小鼠骨髓细胞微核率,采用单细胞凝胶电泳实验检测小鼠外周血淋巴细胞的DNA损伤。结果虾青素干预能抑制辛硫磷染毒所致的小鼠骨髓细胞微核率及外周血淋巴细胞拖尾率和DNA损伤专用单位的升高;且随着虾青素剂量的升高,小鼠的骨髓细胞微核率、外周血淋巴细胞的拖尾率和DNA损伤专用单位均呈下降趋势。结论虾青素对辛硫磷所致小鼠骨髓细胞微核率性的升高及外周血淋巴细胞的DNA损伤具有明显的拮抗作用。Objective To explore the antagonistic effect of astaxanthin on the genotoxicity of phoxim to mice. Methods A total of 50 Kunming mice were randomly divided into five groups as the negative control group, 80 mg/kg body weight phoxim exposure group,low dose,moderate dose and high dose astaxanthin protection groups. In the morning,low dose, moderate dose and high dose astaxanthin protection groups were given astaxanthin by gavage at doses of 2,4 and 8 mg/kg body weight,negative control group and phoxim exposure group were given olive oil. About 8 h later,phoxim exposure group and astaxanthin protection groups were given phoxim by garage at doses of 80 mg/kg body weight in the afternoon for fourteen days, the negative control group were given normal saline. Bone marrow micronuclei test and single cell gel eleetrophoresis were employed to test the genotoxieity. Results The different concentrations（2,4 and 8 mg/kg）of astaxanthin may decrease the rate of mieronuclei and the DNA damage of peripheral lymphoeytes of mice treated with phoxim at 80 mg/kg. Conclusion Astaxanthin has obvious antagonistic effect on the DNA damage of mice induced by phoxim.

关 键 词：虾青素 辛硫磷 DNA损伤 微核实验 单细胞凝胶电泳实验 

分 类 号：R994.6[医药卫生—毒理学]