氮元素对地木耳脂肪酸的影响  

Effect of nitrogen on fatty acids composition of Nostoc commune

作　　者：马飞[1,2] 许璞[2] 朱建一[2] 万能[2] 汤俊[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《食品工业科技》2013年第5期89-92,共4页Science and Technology of Food Industry

基　　金：十二五国家支撑计划(2011BAD13B0902);江苏省科技支撑项目(BE2012416);海洋公益性行业科研专项经费(201105023)

摘　　要：采用改进的Bligh-Dyer(mBD)法,气相色谱-质谱联用法(GC-MS)对含氮(BG11)和无氮(BG110)条件下培养的地木耳(Nostoc commune)脂肪酸进行提取、分离和测定。结果显示:两种培养条件下的地木耳脂肪酸都以C16和C18脂肪酸为主,而且不饱和脂肪酸含量均高于50%。无氮培养条件下地木耳总脂肪酸含量为13.29mg/g,饱和脂肪酸占36.91%,不饱和脂肪酸占63.09%,其中C18∶1的含量为15.99%;含氮条件下培养的地木耳总脂肪酸含量为7.72mg/g,饱和脂肪酸占47.91%,不饱和脂肪酸占52.09%,其中C18∶1的含量为1.74%。结论:无氮培养地木耳的脂肪酸含量显著高于含氮培养组(p<0.05),表明氮源缺失有利于地木耳脂肪酸特别是不饱和脂肪酸的积累。Nostoc commune cultivated in two different conditions（with or without nitrogen in medium）were analyzed for their fatty acids composition using a modified Bligh-Dyer method and GC-MS.The results indicated that C16 and C18 were dominant compositions of total fatty acids （TFAs）, among which the contents of unsaturated fatty acids（UFAs） were all above 50% in all tested samples.TFAs concentration of N.commune cultivated in medium without nitrogen （BG110 ）was 13.29mg/g, while that of saturated fatty acids （SFAs）and UFAs were present at 36.91%,63.09% respectively. C18：1 was present at 15.99% in particular; TFAs concentration of N.commune cultivated in medium with nitrogen（ BGll）was 7.72mg/g,while concentrations of SFAs and UFAs were present at 47.91% ,52.09% respectively.Cl8：1 was 1.74%.The present study showed that the contents of TFAs especially UFAs was significantly（p 〈0.05） higher in BGll0 group than in BGll group.This demonstrated that the absence of nitrogen was propitious to the accumulation of fatty acids especially UFAs.

关 键 词：地木耳 氮源 脂肪酸 气相色谱-质谱联用法 

分 类 号：TS201.2[轻工技术与工程—食品科学]