FRAP法微量模型的建立  

Foundation of micro-model for FRAP Assay

作　　者：卢引[1] 尹震花[1] 康文艺[1] 

机构地区：[1]河南大学中药研究所,河南开封475004

出　　处：《食品工业科技》2013年第5期312-314,共3页Science and Technology of Food Industry

基　　金：河南省科技厅重点项目(102102310019)

摘　　要：目的:建立总抗氧化能力评估法FRAP法微量模型。方法:以槲皮素为研究对象,通过单因素考察,建立L9(34)正交实验,对正交实验进行直观分析和方差分析。结果:由正交实验结果得出最佳实验条件为:1倍量的TPTZ溶液(0.3mol/L醋酸钠缓冲液,10mmol/LTPTZ溶液,20mmol/L氯化铁溶液三者按照顺序体积比为10∶1∶1),加入量为200μL,在37℃下反应时间为40min。验证实验结果RSD值为0.082%,表明该方法稳定可靠,本实验以PG、BHA、BHT做阳性对照,结果表明,此模型准确可行。结论:实验结果表明,本次实验建立的FRAP总抗氧化能力评估法准确,用药量小,稳定可行。Objective：To found micro-model for FRAP assay. Methods：The orthogonal experiment was designed according to L9 （34） orthogonal table after determining the single factor exploration using quercetin as antioxidant. Results： By the direct-viewing analysis and variance analysis of the results of the orthogonal experiment, the optimum experiment conditions were confirmed that the amount of TPTZ solution was i time（Sodium acetate buffer was 0.3 mol/L,TPTZ solution was 10mmol/L, and ferric chloride solution was 20mmol/L.The order volume ratio was 10：1：1 ）.The addition volume of FRAP was 200μL and 40min was kept in 37℃. RSD of the evaluation test was 0.082%,and it showed the method was stable.PG, BHA and BHT were used as standard control,the model was further certified that it was precision and feasibility.Conclusion：The results indicated that the micro-model for FRAP assay from quercetin was accurate,little sample and reliable.

关 键 词：FRAP 抗氧化 微量模型 

分 类 号：TS207.3[轻工技术与工程—食品科学]