霍山石斛多糖对四氯化碳致急性肝损伤小鼠的保护作用  

Protective effects of polysaccharides from Dendrobium huoshanense on CCl_4-induced acute liver injury in mice

作　　者：黄静[1] 李胜立[2] 赵宏伟[2] 潘利华[2] 孙撬撬[2] 罗建平[2] 

机构地区：[1]合肥工业大学校医院,安徽合肥230009 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《中国中药杂志》2013年第4期528-532,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(20872024);合肥工业大学大学生创新实验计划项目(2011CXCY385)

摘　　要：目的:研究霍山石斛多糖对四氯化碳致急性肝损伤小鼠的保护作用。方法:80只小鼠随机分为8组,分别为正常对照组、模型对照组、右旋糖酐对照组、淀粉对照组、多糖全水解产物对照组、霍山石斛多糖高、中、低(200,100,50 mg.kg-1)剂量组,每组10只,各给药组预防给药15 d,第15天给药结束后灌胃50%(用橄榄油稀释)的CCl4(1.5 mL.kg-1BW),建立急性肝损伤模型,连续给药2 d,每天1次。测定小鼠血清ALT和AST及肝匀浆中SOD,MDA含量,免疫组化法观察肝细胞中TNF-α表达水平,HE染色观察肝脏病理组织学变化。结果:霍山石斛多糖各剂量组均能降低CCl4致小鼠急性肝损伤血清ALT,AST的升高,降低肝匀浆中MDA含量,增强SOD的活性,抑制肝细胞中TNF-α表达,减轻CCl4对肝组织的病理损伤。结论:霍山石斛多糖对CCl4致小鼠急性肝损伤具有一定的保护作用,其保护机制可能与清除自由基,抑制脂质过氧化,抑制TNF-α表达有关。Objective：To study the protective effects of polysaccharides from Dendrobium huoshanense（DHP）against CCl4-induced liver injury in mice.Method：Eighty male Kunming mice were randomly divided into normal control group,model control group, destran control group,starch control group,starch control group,three different dose of DPH groups consisting of highdosage group, middle-dosage group and low-dosage group（200,100,50mg·kg-1）.Each group contained ten mice. The mice were treated with DHP via intragastric administration for 15 days before treatment of 50% CCl4 in olive oil for consecutive two days. Both alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities in serum and superoxide dismutase （SOD） activities and malondialdehyde （MDA） contents in liver tissues were determined in all groups . Immunohistochemistry was used to detect the expression of TNF-α in hepatic tissue. Hepatic histopathological examination was observed. Result：DHP effectively decreased the activities of ALT and AST in serum and the contents of hepatic MDA, and restored hepatic SOD activities in acute liver injury mice. Liver tissue damage induced by CCl4 was ameliorated in mice with DHP administration through histopathology examination. Furthermore, the expression of TNF-α was greatly decreased in groups treated with polysaceharides. Conclusion：DHP has a significantly hepatoprotective effect on CCll4-induced acute liver injury in mice. Protective effect of DHP on the liver may be related to its function of scavenging free radicals and inhibiting lipid peroxidation and TNF-α expression.

关 键 词：霍山石斛多糖 四氯化碳 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]