藤茶抗氧化活性研究  

Antioxidant Activity of Ampelopsis grossedentala

作　　者：欧贤红[1,2] 叶勇[3,4] 黄秋洁[5] 刘华钢[3] 宋云飞 

机构地区：[1]泸州医学院,泸州646000 [2]桂林医学院药学院,桂林541004 [3]广西医科大学药学院,南宁530021 [4]桂林莱茵生物科技股份有限公司博士后工作站,桂林541100 [5]广西中医药大学药学院,南宁530001

出　　处：《天然产物研究与开发》2013年第2期245-248,共4页Natural Product Research and Development

基　　金：广西教育厅基金项目(201010LX340)

摘　　要：本文建立体外二苯代苦味酰基自由基(DPPH.)体系,并采用连苯三酚法在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-.)和邻二氮菲-Fe2+/H2O2体系产生羟自由基(.OH)法,在酶标仪上检测藤茶总黄酮、二氢杨梅素和杨梅素对DPPH.、O2-.和.OH的清除作用。结果表明:藤茶各提取物均有不同程度的体外抗氧化效果,杨梅素对DPPH.的清除作用最强DPPH.IC50(2.91±0.28)mg/L,二氢杨梅素对O2-.的清除作用最强IC50(3.88±0.99)mg/L,对.OH的清除效果是杨梅素>二氢杨梅素>藤茶总黄酮。确定杨梅素和二氢杨梅素为藤茶的主要抗氧化相关活性成分。In this study, the scavenging effect of total flavonoid, dihydromyricetin and myrieetin of Ampelopsis grossedntata on DPPH , OH, and 02 were investigated using ELISA Reader. The superoxide free radical （ 02was generated with auto-oxidation in alkalcsent solution with the help of pyrogallol and the hydroxyl free radical （ OH） was produced with 1,10-phenathroline-Fe2/H202. The results showed that the activity of myricetin was the strongest in DPPH. sys- tem （DPPH IC50 2.91 ±0. 28 rag/L）,and the activity of dihydromyricetin was the strongest in O system （ICm 3.88 ± 0.99 rag/L）. In OH system, myricetin displayed the strongest activity,followed by dihydromyricetin and the total flavonoid. Hence, dihydromyricetin and myrieetin may be the main antioxidant components of A. grossedntata.

关 键 词：藤茶 二氢杨梅素 杨梅素 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学]