VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响  

Expression and Significance of VEGF in Salivary Adenoid Cystic Carcinoma Cells in Association with VEGF-ASODN Transfection

作　　者：李晓光[1] 李腾宇[2] 王旭霞[3] 王延秀[4] 肖长杰[1] 徐晓[4] 李俊福[3] 

机构地区：[1]泰安市中心医院口腔科,山东泰安271000 [2]青岛大学医学院口腔系,山东青岛266071 [3]山东大学口腔医学院,山东济南250012 [4]泰安市中心医院麻醉科,山东泰安271000

出　　处：《口腔颌面外科杂志》2013年第1期18-22,共5页Journal of Oral and Maxillofacial Surgery

基　　金：山东省科学技术发展计划资助项目(2011GGH21822)

摘　　要：目的:研究血管内皮细胞生长因子(VEGF)-反义寡核苷酸(ASODN)转染涎腺腺样囊性癌(salivany adenoidcystic cancinoma,SACC)ACC-2细胞对其VEGF表达和生长的抑制作用。方法:人工合成硫代磷酸化VEGF-ASODN,转染SACC-2细胞,24 h后原位杂交及免疫组织化学法检测细胞VEGF mRNA及蛋白表达,ELISA法检测培养液上清中VEGF蛋白含量,流式细胞仪检测细胞凋亡情况,用MTT实验检测转染对细胞活性的影响。结果与对照组相比,以SPSS 13.0软件进行统计学检验。结果:VEGF-ASODN能显著降低VEGF mRNA及蛋白水平、降低培养液中VEGF蛋白含量、增加细胞凋亡、抑制细胞活性及生长。结论:VEGF-ASODN转染SACC-2细胞能显著抑制VEGFmRNA及蛋白表达、增加细胞凋亡、抑制细胞生长。Objective： The study was designed to observe whether VEGF-antisense oligonucleotide （VEGF-ASODN） transfection to SACC-2 cells are associated with its expression of vascular endothelial growth factor （VEGF） and its＇ pathological significances. Methods： The VEGF-ASODN was synthesized artificially with phosphorothioic acid. After transfecting with VEGF-ASODN into SACC-2, the quantity of VEGF mRNA and protein was detected by in situ hybridization and immunohistochemistry. The quantity of VEGF protein in supernatant was detected by ELISA. Flow Cytometry and MTr assay were used to detect cell activity and cellular apoptosis. SPSS 13.0 software was used for statistical analysis. Results： VEGF mRNA protein levels within the cells and in culture medium both decreased significantly after transfected by VEGF-ASODN （P〈0.01 ,P〈0.05）. The ratio of SACC-2 cell apoptosis was increased. The activities of cells were also decreased after transfected by VEGF-ASODN （P〈0.01）. Conclusion： Transfection with VEGFASODN in SACC-2 can markedly inhibit expression of VEGF. It can enhance cellular apoptosis and suppress pathological growth of these cells.

关 键 词：血管内皮细胞生长因子 反义寡核苷酸 转染 涎腺腺样囊性癌ACC-2细胞 

分 类 号：R739.8[医药卫生—肿瘤]