白腐菌Trametes hirsuta SYBC-L19液态发酵水葫芦产漆酶  

Study of Trametes hirsuta SYBC-L19 Liquidstate Fermentation for Laccase Production

作　　者：刘文华[1] 蔡宇杰[1] 孙付宝[1] 张峰[1] 孙啸[1] 范晶晶[1] 廖祥儒[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2012年第12期1252-1261,共10页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2010AA101501);国家重大科技支撑与自主创新专项引导资金项目(BY2010117)

摘　　要：利用单因素法和响应面法对白腐菌Trametes hirsuta SYBC-L19利用水葫芦液态发酵产漆酶的培养基和培养条件进行了优化,确定了最佳培养基:水葫芦41.0 g/L,玉米浆粉50.8 g/L,葡萄糖30.0 g/L,CuSO4.5H2O 0.6 g/L,吐温-80 0.5 g/L,香兰素0.5 mmol/L,无机盐混合液50 mL/L。最佳培养条件为:培养温度30℃、初始pH值4.0、装液量80 mL/250 mL、接种体积分数8%,200r/min摇瓶培养8 d,酶活性达7 784 U/L,是优化之前的17.3倍。该研究为水葫芦等生物质资源的有效利用提供了参考。In this study,the nutritional and environmental conditions of white-rot fungi,Trametes hirsuta SYBC-LI9,for laccase production were investigated and optimized though single factor experiments and response surface methodology. The optimum conditions described as follows ：water hyacinth （dry weight） 41.0 g/L,corn steep powder 50.8 g/L,glucose 30.0 g/L,CuSO4@5H20 0.6 g/L, tween -80 0.5 g/L,vanilline 0.5 mmol/L,inorganic salt mixture 50 mL/L,inoeulation concentration 8%, pH 4.0,liquid volume 80 mL/250 mL,culture temperature 30 ℃. With the conditions,the activity of laccase was achieved at 7 784 U/L,was higher 17.3 times than that of control. The study provides a path and a certain theoretical basis for effective utilization of the water hyacinth and other biomass resource.

关 键 词：TRAMETES hirsuta SYBC-L19 水葫芦 漆酶 优化 响应面 

分 类 号：TQ925[轻工技术与工程—发酵工程]