质量法和HPLC法相结合检测酶解后烟梗果胶质质量分数  

Detecting the Pectin in the Tobacco Stems after Enzymatic by Combing the Gravimetric Method with HPLC

作　　者：施林燕[1] 许赣荣[1] 金永明[2] 汤朝起[2] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]上海烟草集团有限责任公司,上海200082

出　　处：《食品与生物技术学报》2012年第12期1325-1330,共6页Journal of Food Science and Biotechnology

基　　金：上海烟草集团有限责任公司科技项目(SZBC W2010 00744)

摘　　要：将质量法和高效液相色谱法相结合,建立了适合于酶解后烟草样品中果胶质质量分数的检测方法。首先对酶解后的烟梗及梗丝样品进行酸解、过滤处理,所得到的固态样品的果胶酸用质量法检测,原质量法中被丢弃上清液中的半乳糖醛酸质量分数则采用HPLC法检测,将两种方法测定并换算得到的果胶质质量分数相加。结果显示:用HPLC法检测时,半乳糖醛酸检测结果的变异系数为0.33%,平均回收率为99.32%。该法测得的总果胶酸质量分数,其变异系数比用传统质量法检测果胶酸质量分数所得的变异系数有所降低。该方法果胶质提取更充分,酶解后烟梗及梗丝中各种形态的果胶质都能得到检测,具有检测结果更准确、方法简单、便于实际操作的优点。It combined the gravimetric method with HPLC to detect different kinds of pectin in the tobacco stems and cut-stems after enzymatic. Firstly ,the sample of tobacco stems and cut-stems after enzymatic should be treated by acid hydrolysis and fiheration. Then we detected the content of calcium peetinate in the solid samples by gravimetric method. The galacturonic acid in the supernatant that was discarded in the process of sample treatment will be tested by HPLC. These two parts of the content of pectin could be conversed and added together. The analytical results showed that the coefficient of variation was 0.33% ,the average recovery of galacturonic acid was 99.32%.Using this method,the coefficient of variation of the content of the total pectin acid was lower than traditional gravimetric method.The pectin would be fully extracted.The detection method is comprehensive and with good reproducibility ,and easy operations.

关 键 词：烟草 果胶质 质量法 高效液相色谱法 果胶酸 半乳糖醛酸 

分 类 号：TS47[农业科学—烟草工业] O657.72[理学—分析化学]