刺梨籽油的脂肪酸组成和抗氧化活性研究  

Constituents and Antioxidant Activity of Fatty Acids in Seed Oil of Rosa roxburghii

作　　者：史亚男[1,2] 王道平[2] 马琳[2] 游泽芳[2] 杨小生 

机构地区：[1]贵州师范大学化学材料与科学学院,贵州贵阳550002 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《山地农业生物学报》2013年第1期24-28,共5页Journal of Mountain Agriculture and Biology

基　　金：国家自然科学基金"多取代1-羟基-2-吡咯烷酮的合成及钙拮抗活性研究"(30973620);贵州省科技厅基金项目[黔科合J字(2011)2310号]

摘　　要：利用GC-MS联用技术,结合脂肪酸甲酯化反应方法,对刺梨籽油中总脂肪酸、结合型脂肪酸和游离型脂肪酸进行分析测试,并用峰面积归一化法测定各成分相对含量。此外,还通过Fenton体系、DPPH-两种分析方法测定刺梨籽油体外清除自由基能力。结果表明:(1)脂肪酸成分为6种,主要成分为不饱和脂肪酸亚油酸和亚麻酸;(2)刺梨籽油脂中结合型脂肪酸和游离型脂肪酸的比例为1.3∶1(重量比),以亚油酸和亚麻酸为主的游离型脂肪酸7种,以亚油酸和9-十八碳烯酸为主的结合脂肪酸8种;(3)抗氧化活性试验结果表明,刺梨籽油对DP-PH自由基的清除率具有量效关系。当刺梨籽油的浓度为2 mg/mL时,对羟基自由基的清除率为96.82%,也显现量效关系。因此,刺梨籽油可作为一种富含不饱和脂肪酸的功能性食用油脂来深度开发利用。The total fatty acids, combined and free types fatty acids were analyzed by GC-MS technique and methylation reaction method. Besides, The antioxidant activities of the seed oil were also dtermined by Fenton and DPPH free radical （ DPPH · ） method. Results showed （ 1 ） six kinds of fatty acids were detected in seed oil of R. Roxburghii, among which the main components are the unsaturated fatty acids ; （2） the ratio of combined and free types fatty acids was 1.3： 1, the major component of seven kinds of free acids were linolic acid and linolenic acid, and the major component of eight kinds of combined type were linolic acid and 9-oc- tadecenoic acid; and （3） seed oil had dosage effect on hydroxyl radical scavenging, The hydroxyl radical scavenging rate was 96.82% as the usage of 2 mg/mL seed oil. Therefore, seed oil of Rosa Roxburghii may be used as functional edible oil due to high unsaturated fatty acid.

关 键 词：刺梨籽油 结合型脂肪酸 游离型脂肪酸 抗氧化活性 

分 类 号：TS225[轻工技术与工程—粮食、油脂及植物蛋白工程]