HPLC-ELSD法同时测定无糖食品中糖和糖醇的含量  

Determination of Sugar and Sugar Alcohol in Sugar Free Foods by HPLC-ELSD

作　　者：赵志磊[1] 张玉[2] 闫正[2] 谢飞[1] 李小亭[1] 

机构地区：[1]河北大学质量技术监督学院,河北保定071002 [2]河北大学化学与环境科学学院,河北保定071002

出　　处：《食品研究与开发》2013年第3期59-62,共4页Food Research and Development

基　　金：质检公益性行业专项课题资助(200810432)

摘　　要：建立高效液相色谱法同时分离测定无糖食品中多种糖和糖醇的含量。样品提取净化后,采用高效糖分析色谱柱分离(250 mm×4.6 mm;5μm)、乙腈/水梯度洗脱,流速:1.4 mL/min;柱温:35℃;蒸发光散射检测器检测,漂移管温度80℃,喷雾器温度34.8℃,进样量20μL。结果表明:木糖、木糖醇、果糖、山梨醇、葡萄糖、蔗糖、麦芽糖醇、乳糖、乳糖醇进样量1 mg/mL^10 mg/mL的范围线性良好,相关系数为0.994 6~0.999 3;RSD<2%,回收率平均在89.2%~110.9%之间。本方法该方法灵敏度高,重复性好,可同时对无糖食品中的4种糖醇和5种糖进行分离与检测。A quantitive assay for the determination of sugar and sugar alcohol in the sugar free foods was established by high performance liquid chromatography （HPLC）. Sample was extracted and the separation was performed with high performance carbohydrate column （250mm×4.6mm;5μm）, acetonitrile/water as mobile phase. Flow rate：1.4 mL/min;column temperature：35 ℃ ;Evaporative Light Scattering detector conditions： drift tube temperature： 80℃ ,atomizer temperature： 34.8 ℃, sample volume 20 μL. The result showed that the xylose,xylitol,fructose,sorbitol,glucose,sucrose,mahitol,lactose,lactitol showed distinct linearity relation ship with the concentration in the range of 1mg/mL-10 mg/mL, the correlation coefficient was 0.994 6-0.999 3, RSD was 〈2 %, the recovery was 89.2 %-110.9 % . The method was accuracy, sensitivity and feasibility to separate and determinate the sugar and sugar alcohol in sugar free foods.

关 键 词：HPLC—ELSD 糖 糖醇 无糖食品 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]