Correlation between pathogenesis of dampness syndrome and Interleukin-2,Interleukin-8 in rats  

作　　者：Xiaolin Chen Youwu Lin Shijun Zhang Zexiong Chen Carlini Fan Hardi Ting Xiang Baoguo Sun 

机构地区：[1]Department of Traditional Chinese Medicine, The First Affiliated Hospital of SunYat-Sen Universty

出　　处：《Journal of Traditional Chinese Medicine》2013年第1期114-118,共5页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China (No. 81072806);"Eleventh Five-year Plan" for Sci &Tech Research of China (No. 2009ZX10005-016)

摘　　要：OBJECTIVE： To explore the pathogenesis of dampness syndrome by detecting the changes of inter- leukin-2 （IL-2） and interleukin-8 （IL-8） levels. METHODS： Female Sprague Dawley rats were divided randomly into five groups according to the random number table： a normal group （Group Ⅰ ）, an external dampness group （Group Ⅱ ）, an internal dampness group （Group Ⅲ）, and an external and internal dampness group （Group Ⅳ）. Twenty days after the model made, IL-2 and IL-8 levels were detected by radioimmunoassay method. RESULTS： The IL-2 and IL-8 levels among groups were significant （F=3.102, P〈0.05; F=2.657, P〈0.05, respectively）. The level of IL-2 in Group Ⅱ and Group Ⅲ were higher than that in Group Ⅰ （P〈0.05, P〈0.01, respectively）, especially higher in the Group Ⅲ compared with Group Ⅱ （P〈0.05）. The level of IL-8 in Group Ⅲ were higher than those in Group Ⅰ, Group Ⅱ and GroupⅣ （P〈0.05, P〈0.01, P〈 0.05, respectively）. In the Group Ⅲ, the 24-hour water and body weight were higher than that in the Group Ⅱ （all P〈0.05）, and spontaneous movement frequency was higher than those in Group Ⅱ and GroupⅣ （P〈0.05）. CONCLUSION： Immune activation and inflammatory reaction might be easily caused by external danpness other than internal dampness.OBJECTIVE: To explore the pathogenesis of dampness syndrome by detecting the changes of interleukin-2 (IL-2) and interleukin-8 (IL-8) levels. METHODS: Female Sprague Dawley rats were divided randomly into five groups according to the random number table: a normal group (GroupⅠ), an external dampness group (GroupⅡ), an internal dampness group (Group Ⅲ), and an external and internal dampness group (GroupⅣ). Twenty days after the model made, IL-2 and IL-8 levels were detected by radioimmunoassay method. RESULTS: The IL-2 and IL-8 levels among groups were significant (F=3.102, P<0.05; F=2.657, P<0.05, respectively). The level of IL-2 in Group Ⅱ and Group Ⅲ were higher than that in GroupⅠ(P<0.05, P<0.01, respectively), especially higher in the Group Ⅲ compared with GroupⅡ (P<0.05). The lev- el of IL-8 in Group Ⅲ were higher than those inGroupⅠ, GroupⅡ and GroupⅣ (P<0.05, P<0.01, P< 0.05, respectively). In the GroupⅢ, the 24-hour wa ter and body weight were higher than that in the GroupⅣ (all P<0.05), and spontaneous movemen frequency was higher than those in GroupⅡ and GroupⅣ (P<0.05). CONCLUSION: Immune activation and inflammato ry reaction might be easily caused by externa danpness other than internal dampness.

关 键 词：Interior dampness syndrome Excessivedampness syndrome INTERLEUKIN-2 INTERLEUKIN-8 

分 类 号：R228[医药卫生—中医基础理论]