Microbial Transformation of Pregnane-3β,16β,20-triol by Cunninghamella echinulata  

作　　者：Chenru Yang Huafang Fan Yuan Yuan Jinming Gao 

机构地区：[1]Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, College of Science, Northwest Adow University, Yangling, Shaanxi 712100, China

出　　处：《Chinese Journal of Chemistry》2013年第1期127-131,共5页中国化学（英文版）

基　　金：Acknowledgement This work was supported by the National Natural Science Foundation of China （30770237） and the Pro- gram for New Century Excellent Talents in University （NCET-05-0852）.

摘　　要：Microbial transformation of pregnane-3β,16β,20-triol （1） by Cunninghamella echinulata afforded four previ- ously unreported polyhydroxylated steroids, namely pregnane-3β,14a,16β,20-tetrol （2）, pregnane-3-oxo-14a, 16β,20-triol （3）, pregnane-3-oxo-7β,16β,20-triol （4）, and pregnane-3fl,7β,16β,20-tetrol （5）. The structures of these metabolites were established by means of spectroscopic analysis （1D and 2D NMR as well as HRESIMS analysis）.Microbial transformation of pregnane-3β,16β,20-triol （1） by Cunninghamella echinulata afforded four previ- ously unreported polyhydroxylated steroids, namely pregnane-3β,14a,16β,20-tetrol （2）, pregnane-3-oxo-14a, 16β,20-triol （3）, pregnane-3-oxo-7β,16β,20-triol （4）, and pregnane-3fl,7β,16β,20-tetrol （5）. The structures of these metabolites were established by means of spectroscopic analysis （1D and 2D NMR as well as HRESIMS analysis）.

关 键 词：pregnane-3β 16β 20-triol Cunninghamella echinulata biotransformation HYDROXYLATION biocatalysis 

分 类 号：TQ460.38[化学工程—制药化工] TS201.3[轻工技术与工程—食品科学]