ABTS与邻二氮菲-Fe^(3+)法测定保健食品抗氧化能力比较分析  

Comparisons of the Performance of ABTS and(1,10)-2 Phenanthroline-Fe^(3+) in Antioxidant Capacity Measuring

作　　者：刘薇[1] 邱乐[1] 杨婧[1] 刘彦霞[1] 赵建[1] 文镜[1] 

机构地区：[1]北京联合大学应用文理学院生物活性物质与功能食品北京市重点试验室,北京100191

出　　处：《食品工业》2013年第3期120-124,共5页The Food Industry

基　　金：北京市教委科技发展计划项目(KM201011417006)

摘　　要：对ABTS法与邻二氮菲-Fe3+法测定保健食品抗氧化能力的性能进行分析比较。提取样品中抗氧化成分,以水溶性VE(Trolox)为标准,分别用ABTS法和邻二氮菲-Fe3+测定样品的抗氧化能力值。通过对两种方法线性关系,最低检测线,精密度,准确度等指标进行测定,对两种方法进行分析比较。两种方法的平行性,重复性精密度及加标回收率均没有显著差异;ABTS法检测Trolox的灵敏度比邻二氮菲-Fe3+法高一个数量级。ABTS法更适用于水溶性抗氧化混合物的测定,当样品中含有还原电位比Fe3+低的抗氧化成份时,用ABTS法比邻二氮菲-Fe3+法测定的结果更准确。The performances of methods of ABTS and 1,10-2 phenanthroline-Fe^3＋ in determining the antioxidant capacity of water-soluble compounds in functional food was compared. The hydrophilic antioxidants in functional foods were extracted and the antioxidant capacity was detected by 2 methods, and the results were expressed by Trolox-equivalent. The accuracy, linearity, repeatability, recovery rate and limit of detection （LOD） of the 2 methods were studied, and their feasibilities were also verified. Each of the 2 methods showed a good linearity over the Trolox concentration of 0.025-0.4 μmol/L （R^2=l）. The reliability, repeatability and recovery were no differences between the 2 methods. But the method of ABTS reached a lower LOD than the other method. ABTS method is more suitable for the determination the antioxidant capacity of water-soluble antioxidant mixture.

关 键 词：ABTS法 邻二氮菲-Fe3+分光光度法 抗氧化能力 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]