一起食物中毒患者粪便与可疑食品中副溶血性弧菌血清分型与毒力基因检测分析  

Serotyping and detection of virulence genes in Vibrio parahaemolyticus from feces of a food poisoning patient and suspected food

作　　者：张少华[1] 沈飚 王忠发[3] 

机构地区：[1]舟山市中医骨伤联合医院检验科,浙江舟山316000 [2]浙江省检验检疫科学技术研究院舟山分院,浙江舟山316000 [3]舟山市疾病预防控制中心微生物检验科,浙江舟山316021

出　　处：《中华医院感染学杂志》2013年第6期1478-1480,共3页Chinese Journal of Nosocomiology

基　　金：浙江省科技厅项目(2008c32035)

摘　　要：目的进一步了解副溶血弧菌性食物中毒患者粪便与可疑食物中的副溶血弧菌血清型和毒力基因分布。方法细菌分离改用L棒推凃其余参照GB/T4789.7-2003方法,对鉴定为副溶血性弧菌菌株做血清分型和毒力基因(tdh和trh)检测。结果从4份粪便样品和2份从可疑食品中共检出副溶血弧菌28株可分15个血清型,来源于粪便样本的17株副溶血弧菌tdh阳性、trh阴性,来源于可疑食品中的11株副溶血弧菌tdh与trh均阴性。结论该次食物中毒是由一组携带tdh毒力基因多种血清型混合的副溶血弧菌污染引起。OBJECTIVE To further understand the serotypes and virulence genes in Vibrio parahaemolyticus isolated from feces of an food poisoning patient and suspected food. METHODS Bacteria were isolated through petri dish by bent glass rods, the rest isolation steps followed the GB/T4789. 7 2003. Serotyping and virulence genotyping （tdh and trh） were performed for the identified V. parahaemoZyticus strains. RESULTS From four feces specimens and two suspected food specimens, totally 28 V. parahaemolyticus strains with 15 serotypes were identified. Seventeen strains were from feces specimens, all of them were tdh positive and trh negative. The rest 15 strains from the suspected food specimens were both tdh and trh negative. CONCLUSION The contamination of a group of tdh-positive V. parahaemolyticus with mixed serotypes leads to the food poisoning incidence.

关 键 词：食物中毒 副溶血性弧菌 血清分型 毒力基因检测 

分 类 号：R446.1[医药卫生—诊断学]