丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012发酵鲜芭蕉芋生产丁醇  

Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology

作　　者：龙飞[1,2,3] 靳艳玲[1,3] 赵云[4] 郜晓峰[1,2,3] 李宇浩[1,2,3] 陈谦[1,2,3] 赵海[1,3] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]中国科学院环境与应用微生物重点实验室,成都610041 [4]四川大学生命科学学院,成都610064

出　　处：《应用与环境生物学报》2013年第1期54-60,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑计划项目(2011BA D22B03);中国科学院知识创新工程重要方向项目(2011BAD22B03;KSCX2-EW-G-1-1);现代农业产业技术体系项目(CARS-11-A-04)资助~~

摘　　要：以非粮作物鲜芭蕉芋为原料,利用丙酮丁醇梭菌CICC 8012发酵生产丁醇.采用中心组合实验设计(CCD),选取初始糖浓度、接种量、中性红、乙酸铵为主要影响因素,对发酵条件进行优化,建立以丁醇产量为响应值的数学模型.对模型求解得到:初始糖浓度62.25 g/L,接种量为10.81%,中性红为0.81 g/L,乙酸铵为0.574 g/L时,最终的丁醇产量为12.83 g/L.验证实验结果表明,在优化条件下丁醇发酵产量达到12.73 g/L,证明了模型可靠有效.Raw Canna edulis Ker, one of the non-food crops, was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8012. Central Composite Design （CCD） was adopted to optimize experiment conditions including four crucial factors which were initial sugar concentration, inoculation volume, neutral red and ammonium acetate. According to the results of the model, the optimal conditions of butanol fermentation were that： initial sugar concentration 62.25 g/L, inoculation volume 10.81%, neutral red usage 0.81 g/L, ammonium acetate usage 0.574 g/L and final butanol yield of 12.83 g/L. In the verification experiment, the butanol yield of 12.73 g/L was obtained under the optimal conditions, which indicated that the model was reliable and effective. Fig 3, Tab 5, Ref24

关 键 词：丁醇 鲜芭蕉芋 丙酮丁醇梭菌 响应面法 发酵 

分 类 号：TQ116.2[化学工程—无机化工]