光反应酶系统脱木质素作用研究  

Study on delignification in lignocellulose based on enzymolysis stimulated by illumination

作　　者：熊燕飞[1] 甘玮[1] 王洋[1] 李培妹[1] 陈筠[2] 

机构地区：[1]武汉理工大学理学院生物技术系,湖北武汉430070 [2]武汉理工大学校医院化验室,湖北武汉430070

出　　处：《食品工业科技》2013年第7期182-185,189,共5页Science and Technology of Food Industry

摘　　要：利用植物光反应系统的高度氧化活性和光能高效利用机制进行木纤维脱木质素的可行性研究,寻找木纤维脱木质素新方法。从植物光合组织中分离光反应酶系统,观察其对木质素的光促酶降解作用,并观察某些金属离子和有机小分子对光反应酶降解木质素的影响。结果显示,植物光反应酶系统在pH6.0、45℃、7月正午日光照射下,每1mg酶蛋白作用于1.20g构树木纤维,光促酶解3h,木质素降解率达27.7%,残渣纤维素溶出量104.6mg.g^(-1),分别是无光照时的2.9倍和4.4倍,是漆酶的1.9倍和1.6倍。光反应系统的构成离子或电子传递组分及其类似物可提高光反应酶脱木质素效率。即光反应酶系统能有效利用光能降解木纤维木质素,促进纤维素的酶解释放。The feasibility of the high oxidative activity and efficient light energy utilization mechanism of chloroplasts photoreaction system used for lignin degradation had been studied in this paper to look for a new lignin degradation method.Photoreaction enzyme system was separated from plant photosynthetic tissue, and its effect on lignin degradation and its function conditions were investigated.Moreover, the effects of small molecules and illumination on lignin degradation efficiency were investigated.The results demonstrated that Photoreaction enzyme system could effectively degrade lignin in lignocellulose.The appropriate function condition was at pH6.0 and 45℃, and illumination was necessary. In this case, the lignin degradation rate was 27.7% and cellulose dissolution quantity from residue was 104.6mg·g^-1 when 1mg enzyme protein acted on 1.20g paper mulberry lignocellulose for 3h under the sunlight（noon,July） ,which was respectively 2.9 times and 4.4 times than in dark place,and 1.9 times and 1.6 times than those of laccase respectively.Constituention or electronic transfer components or analogue could improve lignin degradation efficiency.In conclusion, photoreaction enzyme could effectively utilize luminous energy to degrade lignin and promote the release of cellulose during the subsequent zymohydrolysis process.

关 键 词：光照 光反应酶系统 木质素 酶促降解 

分 类 号：TS201.2[轻工技术与工程—食品科学]