金黄色葡萄球菌拮抗菌株的筛选及鉴定  

Screening and identification of bacterial strain against Staphyloccocus aureus

作　　者：李成海[1] 贠建民[1] 艾对元[1] 张紊玮[1] 颜东方[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品工业科技》2013年第7期195-198,共4页Science and Technology of Food Industry

基　　金：甘肃省农业生物技术专项(GNSW-2011-18)

摘　　要：从甘肃中部干旱生境土壤中分离筛选到一株对食源性致病菌金黄色葡萄球菌具有抑制作用的拮抗菌,对其发酵液抑菌最低浓度稀释梯度及抑菌谱进行了测定,并通过形态学、生理生化及16SrDNA分子生物学方法鉴定了其种属。结果表明,该金黄色葡萄球菌拮抗菌株还对大肠杆菌、植物酸杆菌和表皮葡萄球菌具有抑制作用,其发酵液对金黄色葡萄球菌抑菌最低浓度稀释梯度为10^(-6),经鉴定,该拮抗菌为产碱杆菌属菌株Alcaligenes sp.。An antagonistic bacterial strain against foodborne pathogen Staphyloccocus aureus was isolated and screened from the drought soil of Gansu central habitat.The minimum inhibitory concentration dilute gradient of the fermentation broth and antibacterial spectrum were determined. The species of the antagonistic bacteria was identified through morphological,physiological and biochemical characteristics and 16S rDNA method.The results showed that the antagonistic bacterial strain against Staphyloccocus aureus also had antagonism against Escherichia coil, Lactobacillus plantarum and Stapyloccocush epidermidis. Its minimum inhibitory concentration dilute gradient against Staphyloccocus aureus was 10^-6 and the antagonistic bacteria was Alcaligenes sp.

关 键 词：金黄色葡萄球菌 拮抗菌 筛选 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]