HPLC-MS法分离鉴定土人参色素组成  

Identification of the pigments from Talinum paniculatum(Jacq.)Gaertn by HPLC-MS

作　　者：陈姗姗[1,2] 常彦[1] 王丹[1] 张超[1] 马越[1] 张晖[2] 赵晓燕[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心、农业部华北地区园艺作物生物学与种质创制重点实验室、农业部都市农业(北方)重点实验室,北京100097 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品工业科技》2013年第7期300-304,共5页Science and Technology of Food Industry

基　　金：北京市自然科学基金(重点项目6111001);现代农业产业技术体系建设专项资金(CARS-25-E-02)

摘　　要：采用液质方法(HPLC-MS),将土人参色素不同组分分离,并对其进行结构鉴定。结果表明:土人参茎和果中色素均为甜菜苷类色素,从土人参茎中分离出五种甜菜苷色素,果中分离出六种,其中五种是二者均有,分别为甜菜苷5-O-β-葡萄糖、甜菜苷6-O-β-葡萄糖、异甜菜苷6-O-β-葡萄糖、2-脱羧基-甜菜苷-6-O-(6’-O-阿魏酰)-β-葡萄糖苷、5,6-二吲哚乙酸-5-O-β-葡萄糖苷。新甜菜苷5-O-β-葡萄糖苷仅存在于土人参果中,在茎中未发现。各组分在土人参茎和果中所占百分数均不同,使二者光谱特征等性质不同。Talinum Crassifolium pigments were tentatively isolated and identified by HPLC- MS.It was concluded that the pigments from Talinum Crassifolium stem and fruit were betacyanins.A total of 6 kinds of betacyanins were isolated and characterized.They were identified as betanidin 5 - O- β- glucoside, betanidin 6- O- β- glucoside, isobetanidin 6- O- β- glucoside, 2- decarboxy- betanid in 6- O- （ 6- O- feruloyl ） - β- g lucoside, 5,6- Dihydroxyindole -5 - O - β- glucoside and neobetanidin 5 - O - β- glucoside, only identified in Talinum Crassifolium fruit. The percentage of betacyanins in Talinum Crassifolium stem and fruit was different, resulting in the different spectral characteristics.

关 键 词：土人参 甜菜苷 液质方法 

分 类 号：TS207.3[轻工技术与工程—食品科学]