方波脉冲电场增强乳酸菌细胞通透性的条件优化  

Optimization of increasing the permeability of Lactobacillus cells by square-wave pulses

作　　者：孙波[1] 赵晓[1] 冀照君[2] 张英华[1] 徐速[1] 马文娟[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]内蒙古民族大学生命科学学院,内蒙古通辽028000

出　　处：《东北农业大学学报》2013年第2期1-6,共6页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅科学技术研究项目(GA06B402-86-6)

摘　　要：利用方波脉冲电场增强乳酸菌细胞通透性,通过将乳酸菌细胞离心,制成浓度为108cfu.mL-1菌悬液,采用不同方波电脉冲条件对其进行处理。结果表明,在0.5 mol.L-1蔗糖的等渗脉冲介质中,电场强度2.25kV.cm-1,脉冲宽度15μs,脉冲数5,间隔时间2 s,此时,乳酸菌细胞的存活率平均为78.24%,细胞内平均荧光强度为5 480。表明脉冲电场可有效增强乳酸菌细胞通透性。In order to increase the permeability of Lactobacillus cells by square-wave pulses. Lactobacillus cells was centrifugated and suspended in the medium with a rather high concentration about 10^8 cfu · mL-1. Suspension of lactobacillus cells was exposed to square-wave electric pulses in different conditions. Results showed that fraction survival cells was 78.24% and fluorescence intensity was 5 480 on average, when we treated the lactobacillus cells under the optimal condition that was electric field strength 2.25 kV.cm-1, pulse durations 15 us, pulse numbers 5, and time interval 2 s, in the isotonic buffer solution with concentration of sucrose （0.5 mol. L-1）. This study indicated that permeability of Lactobacillus cells can be increased by square-wave pulses.

关 键 词：方波脉冲 乳酸菌 通透性 

分 类 号：Q93[生物学—微生物学]