川北地区胃癌差异表达基因的筛选  

Screening of differentially expressed genes in gastric carcinoma in North Sichuan area

作　　者：王朝莉[1] 敬保迁[1] 李丽[1] 冯莉[1] 

机构地区：[1]川北医学院免疫学与分子生物学研究所,南充637000

出　　处：《山西医科大学学报》2013年第3期189-191,共3页Journal of Shanxi Medical University

基　　金：四川省教育厅面上基金资助项目(11ZB158)

摘　　要：目的比较胃癌组织和癌旁正常黏膜组织之间基因表达差异,筛选胃癌特有的差异表达基因片段,为揭示胃癌的病因提供实验室依据。方法运用抑制消减杂交技术(SSH),构建胃低分化腺癌组织cDNA消减文库,采用RT-PCR技术对5例不同阶段胃癌患者的癌组织及癌旁正常黏膜组织相应基因进行筛选。结果成功构建胃低分化腺癌cDNA消减文库,获得5个特异性序列标签(NBPF1、FAM48A、RXFP2、ACTB和QDPR),通过RT-PCR技术进行筛选,获得RXFP2基因的mRNA在所取标本中的差异表达。结论筛选得到胃癌特异性表达基因RXFP2,对了解胃癌发生发展的病因学基础和指导临床治疗具有重要意义。Objective To compare the differential gene expression between gastric carcinoma and normal gastric mucous membrane for screening the differentially expressed genes in gastric carcinoma.Methods The suppression subtractive hybridization（SSH） was used to construct the cDNA subtracted libraries from poorly differentiated gastric adenocarcinoma.RT-PCR was employed to screen the unusual genes from 5 patients at different stages of gastric carcinoma and normal gastric mucous membrane.Results The cDNA libraries from poorly differentiated gastric adenocarcinoma were successfully constructed.Five specificity sequences（NBPF1,FAM48A,RXFP2,ACTB and QDPR） were obtained.RT-PCR showed that RXFP2 mRNA had the differential expression in gastric cancer.Conclusion The screening of gastric cancer specific gene expression of RXFP2 is important to understand the occurrence and development of gastric carcinoma in the etiology of foundation and guide the clinical treatment.

关 键 词：胃癌 SSH RT-PCR 特异基因 

分 类 号：R735.2[医药卫生—肿瘤]