芝麻EST-SSR引物的开发与应用  

Development and application of EST-derived SSR markers for sesame

作　　者：柯涛[1,2] 毛晗[1] 董彩华[1] 陈书元[1] 于景印[1] 赵应忠[1] 刘红艳[1] 刘胜毅[1] 

机构地区：[1]农业部油料作物生物学与遗传育种重点实验室中国农业科学院油料作物研究所,湖北武汉430062 [2]南阳师范学院生命科学与技术学院,河南南阳473061

出　　处：《中国油料作物学报》2013年第1期43-47,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家重点基础研究发展计划(973计划)(2011CB109300);湖北农业科技创新中心;农业部油料作物生物学重点开放实验室开放课题(201202);中国农业科学院油料作物研究所所长基金(1610172011011)

摘　　要：为丰富芝麻EST-SSR引物,本研究从实验室构建的高油芝麻品系中芝14号的不同种子发育期cDNA文库中获得45 569条表达序列标签(expressed sequence tags,EST),通过前处理得到总长17.428Mb的无冗余EST共32 421条。利用MISA和SSR finder软件包工具对EST序列进行SSR位点查找,在这些序列中发现有1 688条Unigene,共有1 949个SSR。共226个序列含2个或2个以上SSR位点。这些SSR中出现频率最高的重复基序类型为三核苷酸(74%)。为开发芝麻EST-SSR引物,探讨EST-SSR用于芝麻品种遗传差异研究的可行性,用12份不同含油量品系材料评价其中34对与油脂代谢相关的基因SSR引物,全部成功扩增,共有3对引物获得多态性条带。结果表明,芝麻EST-SSR标记开发效率较高,对于芝麻品种的鉴定与遗传多样性分析具有很高的应用价值。Currently, the lack of EST sequence information has limited the discovery of sesame EST-SSR primer number. All the 45 569 EST sequences of high oil content sesame species Zhongzhil4 were obtained from a full-length developing seeds eDNA library and assembled 32 421 unigenes. The unigenes represented about 17.428 Mb of genic sequences. Using MISA and SSR finder software to screening SSRs, 1 949 non-redundant （NR） SSRs were identified in 1 688 uniESTs. A total of 226 sequences contained more than one SSR. The trinu-cleotide repeats are the dominant types with the frequency of 74%. All of the designed 34 SSR primer pairs related to oil synthesis genes could amplify characteristic SSR fragments against 12 different oil content species, and 3 primer pairs deal amplified polymorphic fragments. The EST-SSR markers of sesame are highly efficiency, and should be the itools for studies of genetic variation, genetic diversity, and development of genetic linkage maps of sesame.

关 键 词：芝麻 EST-SSR 油脂代谢 SSR标记 

分 类 号：S565.303[农业科学—作物学]