预处理对丝素促进293T细胞生长的影响  

Effects of pretreated silk fibroin on growth of 293T

作　　者：赵培[1,2] 庞广昌[1,2] 王雪青[1,2] 田强[1,2] 姚远[1,2] 王菲[1,2] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津商业大学天津市食品生物技术重点实验室,天津300134

出　　处：《天津师范大学学报（自然科学版）》2013年第1期83-87,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市科技攻关计划重点资助项目(06YFBZNC04200);天津商业大学青年基金资助项目(090109)

摘　　要：采用多种处理方法对丝素进行预处理,探讨其作为支架对293T细胞生长的促进作用.正交实验结果显示:以脱胶方式和包埋方法对结果的影响明显,并有统计学意义(P≤0.01);在经胰蛋白酶脱胶、盐酸作用、以壳聚糖/聚乙二醇包埋的双股丝素蛋白纤维支架上,293T细胞数最多;在经胰蛋白酶脱胶、氢氧化钠作用、以壳聚糖/聚乙二醇包埋的3股丝素蛋白纤维支架细胞上,293T细胞活性达到最大.处理后的支架能使293T细胞很好地生长增殖,细胞呈球状体、集落性附着在丝素蛋白纤维上.Silk was pretreated by varieties of methods, in order to study the effects of the silk fibroin as scaffold on promoting the growth of 293T. The experimental results indicated that the degumming method and embedding method had significant impacts on the growth of 293T, and the difference was significant（P≤0.01 ） by orthogonal design. The biomass of 293T cultured on the double-stranded silk fibroins which were degummed with trypsin, corroded by HC1, embedded with chitosan/PEG was the best, and the activity of 293T cultured on the three-stranded silk fibroins which were degummed with trypsin, corroded by NaOH, embedded with chitosan/PEG was the best. The growth of 293T was promoted by the pretreated silk. Those cells exhibited a spherical shape, and were adhesived as colonies on the silk fibroins.

关 键 词：丝素蛋白 293T细胞 生物相容性 细胞活力 丝素支架 正交实验 

分 类 号：Q249[生物学—细胞生物学]