Cloning of Acetyl-CoA Carboxylase accD Subunit Gene from Brassica campestris and Effect of Its Expression on Oil Content  

云南小油菜accD基因克隆及其表达对含油量的影响(英文)

作　　者：龚莹[1,2] 彭少丹[3] 曲晓言[1] 陈升位[1] 王学军[4] 官春云[4] 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南大学滇池学院,云南昆明650228 [3]云南农业大学农科基础课程实验教学中心,云南昆明650201 [4]湖南农业大学农学院,湖南长沙410128

出　　处：《Agricultural Science & Technology》2013年第2期206-210,234,共6页农业科学与技术（英文版）

基　　金：Supported by the National Key Technology Research and Development Program(2011BAD35B04);the National High Technology Research and Development Program of China (863 Program, 2011AA10A104)~~

摘　　要：[Objective] This study aimed to clone accD gene from Brassica campestris （Yunnan small rapeseed） with different oil content and analyze the effect of accD gene expression level on rapeseed oil content. [Method] The accD gene encoding β-CT Ⅱsubunit of acetyl-CoA carboxylase was cloned from Yunnan small rapeseeds with different oil content. Then, sequence alignment and the influence upon seed oil content by the expression of accD gene were analyzed. [Result] There were a few base pair substitutes among the accD genes from different Yunnan small rapeseeds, and their homology was up to 98.6%. The expression of accD gene was increased with reproduction development, and reached peak in late-stage siliques. The seed oil content was positively influenced by the expression of accD gene in middle-stage and late-stage siliques. [Conclusion] This study provides a certain theoretical basis for illustrating the molecular mechanism about rapeseed oil content and breeding new high-oil rapeseed cultivars.[目的]克隆研究不同含油量的云南小油菜accD基因,并分析其表达对含油量的影响。[方法]以不同含油量的云南小油菜为材料,克隆了乙酰辅酶A羧化酶Ⅱ的β-CT亚基的编码基因accD,并进行了序列比对分析及其表达对种子含油量影响的研究。[结果]不同含油量的云南小油菜的accD基因发生了极少的碱基替换,其同源性高达 98.6%。accD基因的表达随生殖生长的进程依次增强,在晚期角果中表达最高。在中期角果、晚期角果中accD基因的表达水平影响种子含油量,呈正相关。[结论]该研究结果为解析油菜高含油量的分子机理及其高含油量育种提供了一定的理论依据。

关 键 词：Yunnan small rapeseed accD gene CLONING EXPRESSION Oil content 

分 类 号：S565.4[农业科学—作物学]