细胞连接蛋白基因在胃癌中的表达、诱导及突变研究  

Expression, induction and mutation of connexin genes in human gastric cancer

作　　者：沈守荣[1] 李伏娥[1] 刘洋 施正专 邹益友 张熙纯[1] 汤恢焕 

机构地区：[1]湖南医科大学湘雅医院消化科,长沙410008

出　　处：《中华消化杂志》2000年第5期304-307,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目!(396 70 344)

摘　　要：目的　研究人胃癌基因组中Cx基因的表达、探讨诱导剂作用后Cx基因的表达变化及Cx43编码序列有无突变。方法　Northern杂交、RT PCR和PCR 单链构象多肽分析。结果　 1.发现了在人正常胃黏膜上皮、癌旁组织及胃癌中Cx基因的表达规律 ,明确了Cx基因在人胃癌基因组中的表达谱。 2 .维甲酸 (RA)、二甲基亚砜 (DMSO)对胃癌细胞基因组中Cx43基因有诱导表达作用 ,而其本身表达的Cx46在RA及佛波酯 (TPA)作用后明显减弱或消失。 3 .Cx43基因编码区未发现突变。结论　1.Cx32可能是人胃上皮细胞基因组中维持细胞间隙连接通讯功能的特异表达的Cx基因 ,Cx46可能是胃癌Cx基因表达的一种变异。 2 .Cx43基因在胃癌细胞中有可诱导性。 3 .Cx43基因在人胃癌中表达下调的原因不是其编码区的点突变。 4.Objective To study the expression of connexin (Cx) genes and the effects of a variety of inducers on the expression changes of Cx and mutations of Cx coding sequences in human gastric cancer. Methods Northern Blot, RT PCR and PCR single strand conformational polymorphism were used. Results 1.There were regular expression patterns of Cx genes in human normal gastric epithelium, paracancerous tissues and gastric cancers. 2.RA and DMSO can induce the expression of Cx43 in gastric cancer and decrease of Cx46 expression by RA and TPA induction. 3.There was no any mutation in Cx43 coding sequences. Conclusion 1.Cx32 gene may be a specific gene in genome of gastric epithelium constructing gap junctional intercellular communication. 2.Cx43 is a inducible gene in gastric cancer cells. 3.The down regulation of Cx43 expression is not caused by mutation of Cx coding sequences in human gastric cancers. 4.The authors proposed a hypothesis on the variation of expression of Cx genes in human gastric cancer.

关 键 词：胃癌 Cx基因 表达 基因突变 细胞连接蛋白基因 

分 类 号：R735.2[医药卫生—肿瘤]