蛹虫草Cordyceps militaris JN168产虫草素液态发酵条件的优化  

Optimization of Fermentation Conditions for Cordycepin by Cordyceps militaris JN168

作　　者：岳翠翠[1,2] 沈健增 蔡宇杰[1,2] 廖祥儒[1,2] 罗军侠[3] 张大兵[3] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122 [3]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《食品与生物技术学报》2013年第2期135-141,共7页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2010AA101501);江苏省科技创新与成果转化(重大科技支撑与自主创新专项引导资金)项目(BY2010117)

摘　　要：利用单因素筛选和响应面法对蛹虫草Cordyceps militaris JN168产虫草素的液态发酵培养基进行优化,以确定蛹虫草产虫草素的最佳发酵培养基配方。结果表明,蛹虫草产虫草素的最佳碳源为葡萄糖,最适质量浓度为40 g/L;最佳氮源为牛肉膏,最适质量浓度15 g/L;加入的无机盐及其添加量分别为MgSO40.76 g/L,K2HPO40.63 g/L,CaCl20.66 g/L,Na2HPO40.67 g/L。优化后发酵液中虫草素质量浓度达到633.47 mg/L,是优化前的6倍。The components for cordycepin production by Cordyceps militaris JN168 were optimized with single factor experiment and response surface methodology. The purpose of this study is to determine the best fermentation medium for cordycepin production by Cordyceps militaris JN168. Results showed that glucose and beef extract are the appropriate carbon and nitrogen sources with an optimal concentration of 40 g/L and 15 g/L,respectively. And adding inorganic salt is MgSO 4 , K 2 HPO 4 ,CaCl 2 and Na 2 HPO 4 with an optimal concentration of 0.76 g/L,0.63 g/L,0.66 g/L and 0.67 g/L respectively. The cordycepin concentration in the fermentation broth after optimization is 633.47 mg/L,which is 6 times before the optimization.

关 键 词：蛹虫草 虫草素 发酵 优化 

分 类 号：S567.35[农业科学—中草药栽培]