不同产地五味子质量评价及抗氧化活性研究  

Quality Evaluation and Antioxidant Activity Research of Schisandra chinensis from Various Habitats

作　　者：韩志福[1] 胡高升[1] 李娜[1] 樊星[1] 贾景明[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中药材》2012年第12期1904-1909,共6页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划课题(2011BAI03B04)

摘　　要：目的:测定辽宁省不同产地五味子果实中木脂素、粗多糖及总酚这3类化合物的含量,评价不同产地五味子质量和抗氧化活性。方法:采用反相高效液相色谱法测定五味子醇甲、五味子甲素及五味子乙素的含量。采用Folin-Cicalteu's法、苯酚硫酸法、DPPH.法,分别测定不同产地五味子果实的总酚含量、粗多糖含量及自由基清除率。结果:五味子样品中3种木脂素总含量最高的采自辽阳市(21.75 mg/g);沈阳市沈北新区样品粗多糖含量最高(88.72 mg/g);总酚含量和自由基清除率最高的是来源于本溪市关门山的样品,分别为104.24 mg/g和86.3%。线性关系表明,总酚含量与自由基清除率的相关性高于总木脂素含量与自由基清除率的相关性。不同产地五味子的总酚含量和总木脂素含量分别与自由基清除率的线性回归方程为Y=1.3677X+46.97,R2=0.6869和Y=2.5916X+57.927,R2=0.1747。结论:辽宁省不同产地五味子果实中木脂素、总酚和粗多糖的含量有显著差异;五味子中主要的抗氧化成分为总酚类化合物,而木脂素类化合物与自由基清除率无明显相关。Objective：To determine the contents of lignans,crude polysaccharides（CP） and total phenolic compounds（TPC） of Schisandra chinensis from various habitats in Liaoning province and evaluate their quality and free radical scavenging（FRS） activity.Methods：Contents of schisandrol,deoxyschizandrin and schisandrin B were determined by RP-HPLC.Contents of TPC,CP and FRS activity were determined by Folin-Cicalteu′s,phenol-sulfuric acid and DPPH· method,respectively.Results：Sample from Liaoyang city had the highest contents of lignans（21.75 mg/g）;Sample from Shenbei New district of Shenyang city had the highest contents of CP（88.72 mg/g）;Sample from Guanmenshan district of Benxi city had the highest contents of TPC and FRS activity（26.06 mg/g and 86.3%,respectively）.Linear regression analysis results showed that contents of TPC had higher correlation coefficient with FRS activity than that of lignans.Their linear regression equations were Y=1.3677X＋46.97,R2=0.6869 and Y=2.5916X＋57.927,R2=0.1747 for TPC and lignans with FRS activity,respectively.Conclusion：The contents of lignans,CP and TPC are significantly different from samples collected from various habitats in Liaoning province.The main antioxidative substances are TPC,and lignans have no significant correlation with FRS activity.

关 键 词：五味子 木脂素 总酚 粗多糖 自由基清除率 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]