纳米氧化铁颗粒在体标记大鼠室管膜下区神经前体细胞及MR示踪观察  

In vivo labeling of rat neural progenitor cells in subventricular zone with superparamagnetic iron oxide for tracking of magnetic resonance imaging

作　　者：邵志红[1] 王培军[1] 倪炯[1] 李铭华[1] 高晓龙[1] 赵小虎[1] 

机构地区：[1]同济大学附属同济医院放射科,上海200065

出　　处：《中华医学杂志》2013年第11期807-810,共4页National Medical Journal of China

基　　金：国家自然科学基金（81171297）

摘　　要：目的探讨超顺磁氧化铁纳米颗粒（SPIOs）对成体大鼠室管膜下区（SVZ）神经前体细胞（NPCs）的在体标记及MR示踪成像的可行性。方法通过脑立体定位仪向大鼠（n=7）右侧脑室前角前缘区注射3μl SPIOs（浓度7mgFe／ml），于注射当天开始腹腔注射5一溴脱氧尿嘧啶核苷（BrdU），连续注射1周。所有实验动物均于术后第1、3、7、14天进行MR扫描，第14天MR扫描后行灌注固定取脑，标本分别行普鲁士蓝染色以及普鲁士蓝与BrdU免疫组织化学双重染色。结果在7例实验动物中，注射后SPIOs均大部分位于右侧侧脑室前角前缘区域，仅部分进入脑室系统。矢状位T2＊WI能较好地显示SPIOs经颅顶部注入脑内的路径以及沿吻侧迁移流（RMS）向嗅球（OB）区分布的过程，磁共振伪影适中，示踪成功率达100％。染色结果显示SPIOs能较好地标记NPCs，SVZ、RMS以及OB区平均标记率分别约为75．5％、42．3％、23．6％。结论SPIOs能有效地在体标记成体大鼠SVZ的NPCs,MRI能动态地监测标记后的NPCs沿RMS向OB方向迁移的过程。Objective To explore the feasibility of in vivo labeling of adult rat neural progenitor cells （NPCs） in subventrieular zone （SVZ） with superparamagnetic iron oxide particles （SPIOs） for tracking of magnetic resonance imaging （MRI）. Methods A total of 7 SD rats were stereotaetically injected with 3 μl SPIOs （7 mg Fe/ml） into anterior horn of right lateral ventricle and then 5-bromo-2＇-deoxyuridine （BrdU） was injected intraperitoneally once daily for 1 week. MRI was performed at 1, 3, 7 and 14 days post-injection. After the final MRI scan, all rats were transcardially perfused and their brains removed and fixed. The sections were processed for Prussian blue iron staining and Prussian blue plus BrdU immunohistochemical staining. Results In all experimental animals, SPIOs were predominantly located in the anterior horn of right lateral ventricle and partial SPIOs entered the ventricular system. A needle path and a distribution of SPIOs along rostral migratory stream （RMS） towards olfactory bulb （OB） were depicted at the sagittal view of T2 ＊ WI, moderate MR artifact was visible and SPIOs tracking NPCs were successful （ success rate of 100% ）. The result of staining showed SPIOs labeling NPCs were effective. And the labeling rates were 75.5% , 42. 3% , 23.6% in SVZ, RMS and OB respectively. Conclusion Effective in vivo labeling of adult rat NPCs in SVZ with SPIOs is feasible. And dynamical migration of labeling NPCs along RMS towards OB may be visualized on MRI.

关 键 词：磁共振成像 室管膜 放射性示踪剂 

分 类 号：R741[医药卫生—神经病学与精神病学]