乙酸乙酯萃取法精制蛋黄磷脂的工艺研究  

Purification of egg yolk phospholipids by ethyl acetate extraction

作　　者：刘竹青[1] 陈有亮[1] 

机构地区：[1]浙江大学动物科学学院,杭州310058

出　　处：《中国油脂》2013年第3期55-57,共3页China Oils and Fats

摘　　要：用乙酸乙酯萃取法精制蛋黄磷脂,并用正交试验优化精制工艺。结果表明:在料液比1∶4,精制时间20 min,精制次数5次,精制温度0℃的条件下,精制效果最好,脱脂率达到95%以上,磷脂回收率达到98%以上。精制蛋黄磷脂中磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)含量分别为65.39%和13.05%。精制前后PC与PE含量比分别为5.14∶1和5.01∶1。因此,乙酸乙酯萃取精制能显著提高磷脂类物质的含量,但不改变各种磷脂类物质间的比例。Ethyl acetate was used to purify egg yolk phospholipids. The purfication process was optimized by orthogonal expriment. The results showed that the optimal conditions were as follows：ratio of solid to solvent 1 ： 4, purification time 20 min, purification times 5, purification temperature 0 ~C. Under the optimal conditions, the defatting rate and phospholipids recovery rate were up to 95 % and 98% , respec- tively. The contents of PC and PE in finished phospholipids reached 65.39% and 13.05%, respectively. The content ratios of PC to PE before and after purification were 5.14：1 and 5.01：1 ,respectively. The results suggested that ethyl acetate could improve the content of phospholipids significantly but the ratios of them were not changed.

关 键 词：蛋黄磷脂 精制 乙酸乙酯 

分 类 号：TQ645.96[化学工程—精细化工]