以KDR为启动子的双自杀基因系统诱导结肠癌细胞凋亡的实验研究  

Experimental study on the apoptosis of colon carcinoma cells induced by a double suicide gene system driven by KDR promoter

作　　者：王朝阳[1] 黄宗海[2] 

机构地区：[1]内蒙古医科大学附属医院普外科,内蒙古呼和浩特010051 [2]南方医科大学珠江医院普外科,广东广州510282

出　　处：《中国实验诊断学》2013年第3期413-417,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家863计划项目(2001AA217171);广东省自然科学基金(103072)

摘　　要：目的研究以KDR为启动子的双自杀基因系统CDglyTK诱导结肠癌细胞凋亡的实验研究。方法用联合基因AdKDR-CDglyTK腺病毒转染表达KDR不同的人结肠癌细胞HCT116和LS174T,检测转染效率;RT-PCR检测双自杀基因CDglyTK的表达;用梯度浓度的前药5-FC及GCV处理,MTT法检测双自杀基因系统对HCT116和LS174T的细胞毒性作用;流式细胞术观察细胞凋亡变化;免疫组织化学法检测caspase-3蛋白的表达。结果携带双自杀基因的重组腺病毒载体成功转染结肠癌细胞。RT-PCR结果显示:表达KDR的HCT116细胞能表达目的基因,而不表达KDR的LS174T细胞不表达目的基因。5-FC和GCV对转染腺病毒的HCT116有明显的细胞毒作用而对LS174T细胞不敏感。流式细胞仪结果显示:给予5-FC和GCV后HCT116细胞出现凋亡峰,G0/G1升高、S期下降。Caspase-3表达增强(P<0.01)。结论 KDR启动子驱动的双自杀基因系统CDglyTK能特异性杀伤结肠癌HCT116细胞,KDR可作为结肠癌基因治疗的靶点,并通过增强caspase-3途径诱导细胞凋亡。Objective To study the apoptosis effect induced by a double suicide gene system driven by the KDR pro- moter （KDR-CDglyTK）on the colon carcinoma ceils. Methods Adenovirus （Ad）-mediated fusion gene system （KDR- CDglyTK）on the colon carcinoma infected HCTll6 and LS174T ceils that express KDR was different. The infection ef- ficiency and the expression of CDglyTK in the ceils were detected by RT-PCR. The infected cells were treated with the 5-FC and GCV at different concentrations and the cytotoxie effect by MTT method. Apoptosis in HCTll6 cells was de- tected by flow cytometry. The expression of caspase-3 was studied by immunocytochemieal technique. Results Recom- binant aden;virus vector carried double suicide gene successfully transfected colon cancer cells. RT-PCR results showed that HCT116 cells expressed KDR can express purpose gene,the LS174T cells no expressed KDR did not express pur- pose gene. 5-FC and GCV showed ohvious cell poison effect on HCTll6 celts and did not have cell poison effect on LS174T cells. Flow cytometry showed a apoptotic peak and increase for cell percentage in G0-G1 phase and decrease for percentage in S phase in HCTll6 cell. The expression of easpase-3 was increased（P〈0.01）. Conclusion CDglyTK fu- sion gene system driven by the KDR promoter can specific damage HCT116 colon cancer cells. KDR can be used as colon cancer gene therapy targets. KDR-CDglyTK could induce apoptosis through the enhancement caspase 3 ways.

关 键 词：KDR 启动子 结肠癌细胞 自杀基因 

分 类 号：R735.35[医药卫生—肿瘤]