Identification of Molecular Markers for the Thinopyrum intermedium Chromosome 2Ai-2 with Resistance to Barley Yellow Dwarf Virus  

携带抗黄矮病基因的中间偃麦草染色体 2Ai-2特异的分子标记(英文)

作　　者：张增艳[1] 辛志勇[1] 林志珊[1] 陈孝[1] 王晓萍 

机构地区：[1]中国农业科学院作物育种栽培研究所,农业部作物遗传育种重点开放实验室,北京100081

出　　处：《Acta Botanica Sinica》2000年第10期1051-1056,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：TheStateHigh_TechnologyProject (86 3_Z17_0 5_0 1) ;PostdoctorFoundationoftheStateEducationCommission .

摘　　要：The wheat_ Thinopyrum intermedium addition lines Z1,Z2 contain a pair of Th. intermedium chromosomes 2Ai_2 carrying the gene with resistance to barley yellow dwarf virus (BYDV). Genomic in situ hybridization (GISH) was used to analyze the chromosome constitution of Z1,Z2 by using genomic DNA probes from Th. intermedium and Pseudoroegneria strigosa . The results showed that the chromosome constitution of either Z1 or Z2 composes of 42 wheat chromosomes and two Th. intermedium chromosomes (2Ai_2). The 2Ai_2 chromosome is St_E intercalary translocation, in which the E genomic chromosome segment translocated into the middle region of the long arm of chromosome belonging to St genome. With the genomic DNA probe of Ps. strigosa , the GISH pattern specific to the 2Ai_2 chromosome may be used as a molecular cytogenetic marker. A detailed RFLP analysis on Z1, Z2 and their parents was carried out by using 12 probes on the wheat group 2 chromosomes. Twenty RFLP markers specific to the 2Ai_2 chromosome were identified. Two RAPD markers of OPR16 -350 and OPH09 -1580 , specific to the 2Ai_2 chromosome, were identified from 280 RAPD primers. These molecular markers could be used to assisted_select translocation lines with small segment of the 2Ai_2 chromosome and provide tools to localize the BYDV resistance.小麦_中间偃麦草二体异附加系Z1、Z2具有一对携带抗黄矮病基因的中间偃麦草染色体 2Ai_2。利用中间偃麦草 (Thinopyrumintermedium (Host)BarkwothandDewey)和拟鹅冠草 (Pseudoroegneriastrigosa)基因组DNA作探针 ,对Z1、Z2进行基因组原位杂交分析。结果表明 ,Z1、Z2附加的一对中间偃麦草染色体 2Ai_2为St_E染色体 ,E组染色体片段中间插入易位到St组染色体长臂中间区域。特异的基因组原位杂交 (GISH)图谱可作为 2Ai_2染色体特异的分子细胞学标记。利用小麦 (TriticumaestivumL .)第二部分同源群染色体长臂和短臂上的 12个探针 ,对Z1、Z2及其亲本进行较详细的RFLP分析 ,鉴定出 2 0个 2Ai_2染色体特异的RFLP标记。从 2 80个随机引物中 ,筛选出 2个2Ai_2染色体特异的RAPD标记OPR16 -350 和OPH0 9-1580 。

关 键 词：barley yellow dwarf virus (BYDV) Thinopyrum intermedium addition lines genomic   in situ   hybridization (GISH) RFLP RAPD 

分 类 号：Q943[生物学—植物学]