从葡萄糖一步发酵生产维生素C的前体2-酮基-L-古龙酸研究进展  

Research Progress of Producing 2-keto-L-gulonic Acid From D-Glucose by One-step Fermentation

作　　者：陈策实[1] 尹光琳[1] 

机构地区：[1]中国科学院上海生物工程研究中心,上海200233

出　　处：《生物工程进展》2000年第5期51-55,共5页Progress in Biotechnology

摘　　要：This paper reviewed the research progress of producing 2 keto L gulonic acid, an important precursor of L\|ascorbic acid, from D\|Glucose by constructing recombinant microorganisms.At first, the background of producing 2\|KLG from D\|glucose by tandem\|fermentation and one step fermentation through gene engineering techniques is introduced. Then, how to optimize the expression of 2,5\|DKG reductase and block the side\|pathways by creating mutants in the 2\|keto aldose reductase genes in vitro and in vivo by allelic replacement is focused. At the same time, the protein engineering of 2,5\|DKG reductase is also mentioned. Finally, the future of this research and possibility of application are discussed.\;This paper reviewed the research progress of producing 2 keto L gulonic acid, an important precursor of L\|ascorbic acid, from D\|Glucose by constructing recombinant microorganisms.At first, the background of producing 2\|KLG from D\|glucose by tandem\|fermentation and one step fermentation through gene engineering techniques is introduced. Then, how to optimize the expression of 2,5\|DKG reductase and block the side\|pathways by creating mutants in the 2\|keto aldose reductase genes in vitro and in vivo by allelic replacement is focused. At the same time, the protein engineering of 2,5\|DKG reductase is also mentioned. Finally, the future of this research and possibility of application are discussed.\;

关 键 词：维生素C 2-KLG 生物合成 一步发酵法 葡萄糖 

分 类 号：TQ924[轻工技术与工程—发酵工程]