西达本胺对胃癌细胞株SGC-7901的体外抗增殖作用  

Antiproliferative effect of chidamide on human gastric cancer cell line SGC-7901 in vitro

作　　者：周珊[1,2] 姜藻[3] 顾晓怡[3] 

机构地区：[1]萍乡市人民医院肿瘤内科,江西省337000 [2]东南大学环境医学工程教育部重点实验室 [3]东南大学附属中大医院肿瘤中心

出　　处：《江苏医药》2013年第6期632-635,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81172619);教育部重点实验室开放课题(2011EME010)

摘　　要：目的研究组蛋白去乙酰化酶(HDACs)抑制剂西达本胺对胃癌细胞株体外抗增殖作用及其可能机制。方法用西达本胺终浓度为0、16、32、64、128、256μmol/L(分别为B、A1、A2、A3、A4、A5组)处理SGC-7901细胞24、48、72h。采用MTT法及流式细胞术分别检测细胞增殖及细胞凋亡,反转录PCR(RT-PCR)检测沉默信息调节因子2相关酶1(SIRT1)及HDAC11mRNA表达,实时荧光定量PCR(RT-qPCR)检测表皮生长因子受体(EGFR)mRNA表达,Western blot检测Notch1蛋白的表达。结果与B组相比,西达本胺呈浓度依赖性地抑制SGC-7901细胞增殖(P<0.05),提高SGC-7901细胞凋亡率(P<0.05),阻断SIRT1、HDAC11、EGFR mRNA以及Notch1蛋白表达(P<0.05)。结论西达本胺可抑制SGC-7901细胞增殖,促进细胞凋亡;其作用机制可能是降低SIRT1、HDAC11基因的表达或与Notch及EGFR信号通路相关。Objective To investigate the antiproliferative effect and underlying mechanism ofhistone deacetylase（HDAC） inhibitor chidamide on human gastric cancer cell line SGC-7901 in vitro. Methods SGC-7901 cells were divided into six groups of B （ehidamide 0 μmol/L）, A1 （chidamide 16μmol/L）, A2（ehidamide 32 μmol/L）, Aa（chidamide 64 μmol/L）, A4（ehidamide 128 μmol/L） and A5 （chidamide 256 μmol/L）. The cell proliferation and cell apoptosis were detected by MTT and flowcytometry, respectively. The mRNA expressions of silent mating type information regulator 2 homolog 1（SIRT1） and HDACll were determined by reverse transcription-PCR（RT-PCR）, and the mRNAexpression of epithelial growth factor receptor（EGFR） was measured by real-time quantitative-PCR （RT-qPCR）. The protein expression of Notch1 was analyzed by Western blot. Results Comparedwith group B, chidamide inhibited cell proliferation and promoted cell apoptosis in a dose-dependent manner（P〈0. 05）, and significantly blocked the expressions of SIRT1 mRNA, HDACll mRNA,EGFR mRNA and Notch1 protein （P〈0. 05 ）. Conclusion Chidamide can inhibit SGC-7901 cell proliferation and enhance cell apoptosis probably via decreasing gene expressions of SIRT1 andHDAC11 or correlating with Notch and EGFR signaling pathway.

关 键 词：胃癌 西达本胺 组蛋白去乙酰化酶11 沉默信息调节因子2相关酶1 NOTCH1 表皮生长因子受体 

分 类 号：R735[医药卫生—肿瘤]