一氧化氮处理对采后番木瓜果实乙烯生物合成的影响  

Effects of nitric oxide treatment on ethylene biosynthesis in harvested papaya fruit

作　　者：郭芹[1] 王吉德[1] 李雪萍[2] 陈维信[2] 吴斌[3] 

机构地区：[1]新疆大学化学化工学院,新疆乌鲁木齐830046 [2]华南农业大学园艺学院/广东省果蔬保鲜重点实验室,广东广州510642 [3]新疆农业科学院农产品贮藏加工研究所,新疆乌鲁木齐830091

出　　处：《广东农业科学》2013年第3期75-78,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金-广东省联合基金项目(U0631004);广东省自然科学基金团队项目(06200670);科技部支撑计划项目(2011BAD24B02-4)

摘　　要：为了解一氧化氮(NO)处理对番木瓜果实乙烯生物合成的影响,采用60μL/L NO熏蒸处理采收成熟度为果皮浅绿并微带黄色痕迹的番木瓜果实3 h,然后在20℃和相对湿度为85%条件下贮藏20 d。研究NO对番木瓜乙烯、1-氨基环丙烷-1-羧酸(ACC)、丙二酰-1-氨基环丙烷-1-羧酸(MACC)、ACC合成酶(ACS)和ACC氧化酶(ACO)及CpACS2和CpACO1基因表达的影响。结果表明,NO处理降低了番木瓜果实乙烯释放量、ACO的活性及CpACO1基因的表达,导致贮藏过程果实ACC和MACC的积累,但对ACS的活性及CpACS2基因的表达无显著抑制作用。Effects of nitric oxide （NO） on ethylene biosynthesis in papaya fruit were investigated. Papaya fruit harvested at light green with some hint of yellow at the blossom end, were first fumigated with 60 υL/L NO for 3 h, then stored at 20℃ with 85% relative humidity （RH） for 20 days. Changes in the levels of ethylene, 1-aminocyclopropane-1-carboxylic acid （ACC）, 1- malonylaminoeyelopropane-l-earboxylie acid （MACC）, as well as the activities of ACC synthase （ACS）, ACC oxidase （ACO） and the expression of CpACS2 and CpAC01 genes were measured during papaya ripening. The results showed that NO treatment inhibited ethylene production, ACO activity and the expression of CpA CO1 gene, leading to the accumulation of ACC and MACC during storage. However, ACS activity and the expression of CpA CS2 gene were not suppressed significantly by NO treatment.

关 键 词：番木瓜 一氧化氮 乙烯合成 ACC合成酶 ACC氧化酶 

分 类 号：S371[农业科学—农产品加工]