胎儿膀胱穿刺液细胞培养染色体核型分析  

Karyotype analysis using cells cultured from fetal bladder centesis

作　　者：高健[1] 戎立敏[1] 梅冰[1] 余小平[1] 郭文潮[1] 李亚丽[1] 

机构地区：[1]河北省人民医院,石家庄050051

出　　处：《中华医学遗传学杂志》2013年第2期207-209,共3页Chinese Journal of Medical Genetics

基　　金：河北省科技计划重点项目（11276102D）

摘　　要：目的探讨胎儿膀胱穿刺液细胞培养染色体分析的可行性。方法抽取3例超声检查诊断为巨大膀胱、羊水过少胎儿的膀胱内液体，取胎儿膀胱穿刺液细胞进行体外培养，染色体制备、核型分析。结果3例的细胞培养均获得成功，获得了可供计数及核型分析的染色体分裂相。胎儿1的染色体核型为46，XY；胎儿2的染色体核型为46，XX，t（1；5）（q22；q1217]／46，XX[4]；胎儿3的染色体核型为46，XY。结论胎儿膀胱穿刺液细胞经过体外培养可以用来进行染色体核型分析，使孕周较小、巨大膀胱、羊水过少胎儿的产前染色体检查成为可能，是产前诊断的一种新途径。Objective To explore the feasibility of karyotype analysis using cells cultured from fetal bladder centesis samples. Methods Samples were extracted upon fetal bladder centesis for 3 fetuses featuring giant bladder and oligohydramnios. Following in vitro culture, cells were routinely processed and stained for chromosome analysis. Results For all 3 cases, ceil culture has achieved success. Sufficient metaphase cells were obtained for chromosome counting and karyotype analysis. The karyotypes of the 3 fetuses were respectively46,XY, 46,XX,t（1;5）（q22;q12）[7]/46,XX[4], and 46,XY. Conclusion Cells cultured from fetal bladder centesis may be used for karyotype analysis following in vitro culturing. This new approach can enable prenatal chromosome analysis for fetuses featuring smaller gestational weeks, giant bladder and oligohydramnios.

关 键 词：产前诊断 膀胱穿刺液 细胞培养 染色体核型分析 

分 类 号：R714[医药卫生—妇产科学]